Chlorophyll a fluorescence and growth of Neoregelia concentrica (Bromeliaceae) during acclimatization in response to light levels

  • João Paulo Rodrigues Martins
  • Edilson Romais Schimildt
  • Rodrigo Sobreira Alexandre
  • Antelmo Ralph Falqueto
  • Wagner Campos Otoni
Plant Tissue Culture


Tissue culturing is widely used for the clonal propagation of several plant species. However, high mortality rates may occur during the acclimatization phase due to the sudden, dramatic changes in light levels. This study evaluated the effect of light level during the acclimatization of Neoregelia concentrica (Vellozo) LB Smith using chlorophyll a fluorescence and growth analysis. N. concentrica plants were multiplied and rooted in vitro on Murashige and Skoog medium and then transferred to plastic trays with individual cells containing vermiculite. The treatments consisted of the ex vitro cultivation of plants under light levels of 30, 50, 70, and full sunlight (100% light) for 28 d. Chlorophyll a fluorescence measurements were taken at 0 (in vitro), 7, 14, 21, and 28 d of acclimatization, and growth trait analyses were made at 28 d. The high irradiance under full sunlight (100% light) induced photoinhibition and reduced the growth rate. Plants grown under partial light (30, 50, and 70%) were less inhibited. A 50% reduction in light level during N. concentrica acclimatization produced rapid plant recovery and higher growth rates than full sunlight.


Acclimatization Bromeliad JIP test Photoinhibition Tissue culture 



The authors would like to acknowledge the scholarship awarded by Espírito Santo State Research Foundation (FAPES). The authors are also grateful to Patrick Callegari Magnani for his technical assistance.


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Copyright information

© The Society for In Vitro Biology 2015

Authors and Affiliations

  • João Paulo Rodrigues Martins
    • 1
  • Edilson Romais Schimildt
    • 2
  • Rodrigo Sobreira Alexandre
    • 2
  • Antelmo Ralph Falqueto
    • 3
  • Wagner Campos Otoni
    • 4
  1. 1.Plant Tissue Culture Laboratory, Agriculture DepartmentFederal University of LavrasLavrasBrazil
  2. 2.Plant Breeding Laboratory/PPGATFederal University of Espírito SantoSão MateusBrazil
  3. 3.Plant Ecophysiology LaboratoryFederal University of Espírito SantoSão MateusBrazil
  4. 4.Tissue Culture Laboratory II/BIOAGRO, Plant Biology DepartmentFederal University of ViçosaViçosaBrazil

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