NMR-based metabolomics profile comparisons to distinguish between embryogenic and non-embryogenic callus tissue of sugarcane at the biochemical level
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Nuclear magnetic resonance (NMR)-based metabolomics profile comparisons of embryogenic and non-embryogenic calli of sugarcane were performed using principal component analysis (PCA) to determine a possible relationship between certain metabolites and somatic embryogenesis. Mahalanobis distance (DM) analysis showed significant metabolic profile differences between the embryogenic and non-embryogenic callus groups. Significantly different spectral buckets and their corresponding metabolites have been identified using volcano- and loading-plot analyses, where glucose, fructose, sucrose, and alanine were observed at higher concentrations and asparagine, glutamine, lysine, 2-hydroxyisobutyrate, and choline were observed at lower concentrations in embryogenic calli than in non-embryogenic calli. The results of this research indicate possible roles of different sugars, amino acids, and aliphatic compounds during sugarcane somatic embryogenesis.
KeywordsSugarcane Embryogenic callus Non-embryogenic callus NMR-based metabolomics Pathway analysis
We would like to thank Dr. Jack C. Comstock of USDA-ARS Sugarcane Field Station Canal Point, Florida for supplying the sugarcane materials used for initiating callus cultures that were used in this investigation as plant tissue materials for comparison. AB is supported by SC-INBRE (2 P20 GM103499), BS was supported by BlueCross BlueShield of South Carolina, and IM was supported by Biotechnology graduate program of Claflin University, South Carolina.
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