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Genotyping and metabolite characterization of somatic hybrids between Arabidopsis thaliana and Swertia mussotii

  • Somatic Cell Genetics
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Abstract

Symmetric and polyethylene glycol (PEG)-induced asymmetric fusions between Swertia mussotii and Arabidopsis thaliana protoplasts generated >100 putative hybrid cell lines. Of these, 65 were shown to have a hybrid origin, based on molecular markers, chromosome number, and morphology. An assay directed at the A. thaliana CACTA transposon family detected only three positive lines among the 65 hybrid calli tested. The CACTA sequence amplified from clone D14 was highly homologous with CAC2, indicating that the chromosome content of the hybrid cell lines was largely inherited from S. mussotii. Profiles of secondary metabolites identified a number of S. mussotii- or A. thaliana-specific compounds, as well as significant proportions of compounds not represented in the profile of either parent.

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Acknowledgments

This research was financially supported by the National Key Technology R&D Program of the Ministry of Science and Technology (2011BAI06B01), National High Technology Research and Development Program “863” (2013AA102602-4), National Natural Science Foundation (grant nos. 30970243, 31270328, 31200226, and 31201269), Science & Technology Plan of Shandong Province (2013GNC11010), and Research Program for International S&T Cooperation Projects of Shandong Province (2011176). We thank Dr. Robert Koebner (UK) for English editing of the manuscript.

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    Authors and Affiliations

    1. Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Shanda Nanlu 27#, Jinan, 250100, China

      Yunfei Cai, Taiyong Quan, Yang Yu, Guanghua Liu & Fengning Xiang

    2. School of Life Sciences, Qilu Normal University, Wenbo Lu 2#, Zhangqiu, 250200, China

      Yunfei Cai

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    1. Yunfei Cai
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    2. Taiyong Quan
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    3. Yang Yu
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    4. Guanghua Liu
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    5. Fengning Xiang
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    Correspondence to Fengning Xiang.

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    Editor: Kazuo Watanabe

    Yunfei Cai, Taiyong Quan and Yang Yu contributed equally to this work.

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    Cai, Y., Quan, T., Yu, Y. et al. Genotyping and metabolite characterization of somatic hybrids between Arabidopsis thaliana and Swertia mussotii . In Vitro Cell.Dev.Biol.-Plant 51, 360–368 (2015). https://doi.org/10.1007/s11627-015-9680-2

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