Advertisement

Improved in vitro rooting and acclimatization of Capsicum chinense Jacq. plantlets

  • A. Barrales-López
  • A. Robledo-Paz
  • C. Trejo
  • E. Espitia-Rangel
  • J. L. Rodríguez-De La O
Micropropagation

Abstract

In vitro conditions such as low light intensity, the levels of sucrose and other nutrients, and high relative humidity may cause anatomical and physiological changes that have negative effects on acclimatization of regenerated plants. The management of these conditions during in vitro culture could improve the plant ex vitro performance. The influences of light intensity, sucrose concentration, the nutritional composition of the culture medium, and culture container size on in vitro rooting and acclimatization were evaluated in Capsicum chinense cv. Naranja plants. The size of the culture container vessel did not affect plant growth. The best responses of the growth variables tested (plant height, number of leaves, number of roots, dry weight, and osmotic potential) and of survival and rooting were obtained with a photosynthetic photon flux density (PPFD) of 28 μmol m−2 s−1 and H medium with sucrose supplementation at either 1.5% or 3% (w/v). The combination of a PPFD of 28 μmol m−2 s−1 and H medium with 1.5% sucrose can be used for successful rooting of C. chinense adventitious shoots, to enhance the performance of the plantlets in the greenhouse or field and to reduce production costs. The factors studied during in vitro culture significantly influenced ex vitro growth.

Keywords

Capsicum Habanero pepper Micropropagation Hydroponic solution Arnon and Hoagland solution 

Notes

Acknowledgments

The authors would like to express their gratitude to Consejo Nacional de Ciencia y Tecnología (CONACYT, Mexico) for the scholarship awarded to ABL (175327), Fundación “Czeslawa Prywer Lidzbarska,” and Consejo Mexiquense de Ciencia y Tecnología (COMECYT).

References

  1. Adelberg J, Delgado M, Tomkins J (2007) In vitro sugar and water use in diploid and tetraploid genotypes of daylily (Hemerocallis spp.) in liquid medium as affected by density and plant growth regulators. Hortscience 42:325–328Google Scholar
  2. Ali MB, Hahn EJ, Paek KY (2005) Effects of light intensities on antioxidant enzymes and malondialdehyde content during short-term acclimatization on micropropagated Phalaenopsis plantlet. Environ Exp Bot 54:09–120CrossRefGoogle Scholar
  3. Aragón CE, Escalona M, Capote I, Pina D, Cejas I, Rodríguez R, Cañal MJ, Sandoval J, Roels S, Debergh P, González-Olmedo J (2005) Photosynthesis and carbon metabolism in plantain (Musa aab) plantlets growing in temporary immersion bioreactors and during ex vitro acclimatization. In Vitro Cell Dev Biol Plant 41:550–554CrossRefGoogle Scholar
  4. Arigita L, González A, Sánchez RT (2002) Influence of CO2 and sucrose on photosynthesis and transpiration of Actinidia deliciosa explants cultured in vitro. Physiol Plant 115:166–173PubMedCrossRefGoogle Scholar
  5. Arnon DI, Hoagland DR (1940) Crop production in artificial culture solutions and in soils with special reference to factors influencing yields and absorption of inorganic nutrients. Soil Sci 50:463–483Google Scholar
  6. Bressan W (2002) Factors affecting "in vitro" plant development and root colonization of sweet potato by Glomus etunicatum Becker & Gerd. Braz J Microbiol 33:31–34CrossRefGoogle Scholar
  7. Cisneros-Pineda O, Torres-Tapia LW, Gutiérrez-Pacheco LC, Contreras-Martín F, González-Estrada T, Peraza-Sánchez SR (2007) Capsaicinoids quantification in chili peppers cultivated in the state of Yucatán, México. Food Chem 104:1755–1760CrossRefGoogle Scholar
  8. Correll MJ, Weather PJ (2001) Effects of light, CO2 and humidity on carnation growth, hyperhydration and cuticular wax development in a mist reactor. In Vitro Cell Dev Biol Plant 37:405–413CrossRefGoogle Scholar
  9. Couceiro MA, Afreen F, Zobayed SMA, Kozai T (2006) Enhanced growth and quality of St. John’s wort (Hypericum perforatum L.) under photoautotrophic in vitro conditions. In Vitro Cell Dev Biol Plant 42:278–282CrossRefGoogle Scholar
  10. Deberh PC (1991) Acclimatization techniques of plants from in vitro. Acta Hortic 289:291–299Google Scholar
  11. Dewir YH, Chakrabarty D, Ali MB, Hahn EJ, Paek KY (2005) Effects of hydroponic solution EC, substrates, PPF and nutrient scheduling on growth and photosynthetic competence during acclimatization of micropropagated Spathiphyllum plantlets. Plant Growth Regul 46:241–251CrossRefGoogle Scholar
  12. Estrada-Luna AA, Davies FT, Egilla JN (2001) Physiological changes and growth of micropropagated chile ancho pepper plantlets during acclimatization and post-acclimatization. Plant Cell Tiss Organ Cult 66:17–24CrossRefGoogle Scholar
  13. Fal MA, Majada JP, Sánchez-Tamés MR (2002) Physical environment in non-ventilated culture vessel affects in vitro growth and morphogenesis of several cultivars of Dianthus caryophyllus L. In Vitro Cell Dev Biol Plant 38:589–594CrossRefGoogle Scholar
  14. Fuentes G, Talavera C, Desjardins Y, Santamaria JM (2005a) High irradiance can minimize the negative effect of exogenous sucrose on the photosynthetic capacity of in vitro grown coconut plantlet. Biol Plant 49:7–15CrossRefGoogle Scholar
  15. Fuentes G, Talavera C, Oropeza C, Desjardins Y, Santamaría JM (2005b) Exogenous sucrose can decrease in vitro photosynthesis but improve field survival and growth of coconut (Cocus nucifera L.) in vitro plantlets. In Vitro Cell Dev Biol Plant 41:69–76CrossRefGoogle Scholar
  16. Gogoi S, Acharjee S, Devi J (2014) In vitro plantlet regeneration of Capsicum chinense Jacq. cv. ‘Bhut jalakia’: hottest chili of northeastern India. In Vitro Cell Dev Biol Plant 50:235–241CrossRefGoogle Scholar
  17. Hazarika BN (2003) Acclimatization of tissue-cultured plants. Curr Sci 85:1704–1712Google Scholar
  18. Huang C, Chen C (2005) Physical properties of culture vessels for plant tissue culture. Biosyst Eng 91:501–511CrossRefGoogle Scholar
  19. Kehie M, Kumaria S, Tandom P (2012) In vitro plantlet regeneration from nodal segments and shoot tips of Capsicum chinense Jacq. cv. Naga King Chili. 3. Biotech 2:31–35Google Scholar
  20. Kehie M, Kumaria S, Tandom P (2013) In vitro plantlet regeneration from cotyledon segments of Capsicum chinense Jacq. cv. Naga King Chili. Sci Hortic 164:1–8CrossRefGoogle Scholar
  21. Kubota C, Tadokoro N (1999) Workshop on bioreactor technology control of microbial contamination for large-scale photoautotrophic micropropagation. In Vitro Cell Dev Biol Plant 35:296–298CrossRefGoogle Scholar
  22. Le VQ, Samson G, Desjardins Y (2001) Opposite effects of exogenous sucrose on growth, photosynthesis and metabolism of in vitro plantlets of tomato (L. esculentum Mill.) grown under two levels of irradiance and CO2 concentration. J Plant Physiol 158:599–605CrossRefGoogle Scholar
  23. López-Puc G, Canto-Flick A, Barredo-Pool F, Zapata-Castillo P, Montalvo-Peniche MC, Barahona-Pérez F, Santana-Buzzy N, Iglesias-Andreu L (2006) Direct somatic embryogenesis: a highly efficient protocol for in vitro regeneration of habanero pepper (Capsicum chinense Jacq.). Hortscience 41:1645–1650Google Scholar
  24. Lucchesini M, Mensuali-Sodi A (2004) Influence of medium composition and vessel ventilation on in vitro propagation of Phillyrea latifolia L. Sci Hortic 100:117–125CrossRefGoogle Scholar
  25. Manzur JP, Penella C, Rodríguez-Burruezo A (2013) Effect of the genotype, developmental stage and medium composition on the in vitro culture efficiency of immature zygotic embryos from genus Capsicum. Sci Hortic 16:181–187CrossRefGoogle Scholar
  26. Mosaleeyanon K, Cha-Um S, Kirdmanee C (2004) Enhanced growth and photosynthesis of rain tree (Samanea saman Merr.) plantlets in vitro under a CO2-enriched condition with decreased sucrose concentrations in the medium. Sci Hortic 103:51–63CrossRefGoogle Scholar
  27. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479CrossRefGoogle Scholar
  28. Nagel KA, Schurr U, Walter A (2006) Dynamics of root growth stimulation in Nicotiana tabacum in increasing light intensity. Plant Cell Environ 29:1936–1945PubMedCrossRefGoogle Scholar
  29. Nhut DT, Dieu Huong NT, Van Khiem D (2004) Direct microtuber formation and enhanced growth in the acclimatization of in vitro plantlets of taro (Colocasia esculenta spp.) using hydroponics. Sci Hortic 101:207–212CrossRefGoogle Scholar
  30. Nhut DT, Nguyen NH, Thuy DTT (2006) A novel in vitro hydroponic culture system for potato (Solanum tuberosum L.) microtuber production. Sci Hortic 110:230–234CrossRefGoogle Scholar
  31. Nowak J, Sroka S, Matysiak B (2002) Effects of light level, CO2 enrichment, and concentration of nutrient solution on growth, leaf nutrient content, and chlorophyll fluorescence of boston fern microcuttings. J Plant Nutr 25:2161–2171CrossRefGoogle Scholar
  32. Pino J, González M, Ceballos L, Centurion-Yah AR, Trujillo-Aguirre J, Latournerie-Moreno L, Sauri-Duch E (2007) Characterization of total capsaicinoids, colour and volatile compounds of habanero chilli pepper (Capsicum chinense Jacq.) cultivars grown in Yucatán. Food Chem 104:1682–1686CrossRefGoogle Scholar
  33. Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equation for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975:384–394CrossRefGoogle Scholar
  34. Premkumar A, Mercado JA, Quesada MA (2001) Effects of in vitro tissue culture conditions and acclimatization on the contents of rubisco, leaf soluble proteins, photosynthetic pigments, and C/N ratio. J Plant Physiol 158:835–840CrossRefGoogle Scholar
  35. Pruski K, Astatkie T, Mirza M, Nowak J (2002) Photoautotrophic micropropagation of russet burbank potato. Plant Cell Tissue Organ Cult 69:197–200CrossRefGoogle Scholar
  36. Rodríguez R, Cid M, Pina D, González-Olmedo JL, Desjardins Y (2003) Growth and photosynthetic activity during acclimatization of sugarcane plantlets cultivated in temporary immersion bioreactors. In Vitro Cell Dev Biol Plant 39:657–662CrossRefGoogle Scholar
  37. Rolland F, Moore B, Sheen J (2002) Sugar sensing and signaling in plants. Plant Cell 14:185–205Google Scholar
  38. Sanatombi K, Sharma GJ (2008a) In vitro propagation of Capsicum chinense Jacq. Biol Plant 52:517–520CrossRefGoogle Scholar
  39. Sanatombi K, Sharma GJ (2008b) In vitro plant regeneration in six cultivars of Capsicum spp. using different explant. Biol Plant 52:141–145CrossRefGoogle Scholar
  40. Sha Valli Khan PS, Kozai T, Nguyen QT, Kubota C, Dhawan V (2002) Growth and net photosynthetic rates of Eucalyptus tereticornis Smith under photomixotrophic and various photoautotrophic micropropagation conditions. Plant Cell Tissue Organ Cult 71:141–146CrossRefGoogle Scholar
  41. Sha Valli Khan PS, Kozai T, Nguyen QT, Kubota C, Dhawan V (2003) Growth and water relations of Paulownia fortunei under photomixotrophic and photoautotrophic conditions. Biol Plant 46:161–166CrossRefGoogle Scholar
  42. Solís-Ramos LY, Nahuath-Dzib S, Andrade-Torres A, Barredo-Pool F, González-Estrada T, Castaño de la Serna E (2010) Indirect somatic embryogenesis and morphohistological analysis in Capsicum chinense. Biologia 65:504–511CrossRefGoogle Scholar
  43. Talavera C, Contreras F, Espadas F, Fuentes G, Santamaría JM (2005) Cultivating in vitro coconut palms (Cocos nucifera L.) under glasshouse conditions with natural light, improves in vitro photosynthesis nursery survival and growth. Plant Cell Tissue Organ Cult 83:287–292CrossRefGoogle Scholar
  44. Valadez-Bustos MG, Aguado-Santacruz GA, Carrillo-Castañeda G, Aguilar-Rincón VH, Espitia-Rangel E, Montes-Hernández S, Robledo-Paz A (2009) In vitro propagation and agronomic performance of regenerated chili pepper (Capsicum spp.) plants from commercially important genotypes. In Vitro Cell Dev Biol Plant 45:650–658CrossRefGoogle Scholar
  45. Ventura-Zapata E, Salcedo-Morales G, Hernández-Lauzardo AN, Martínez-Bonfil B, Trejo-Tapia G, De Jesús-Sánchez A, Velázquez-Del Valle M, Jiménez-Aparicio A (2003) In vitro regeneration and acclimatization of plants of turmeric (Curcuma longa L.) in a hydroponic system. Biotechnol Appl 20:25–31Google Scholar
  46. Zobayed SMA, Afreen F, Kubota C, Kozai T (2000) Water control and survival of Ipomoea batatas grown photoautrophically under forced ventilation and photomixotrophically under natural ventilation. Ann Bot 86:603–610CrossRefGoogle Scholar
  47. Zobayed SMA, Afreen-Zobayed F, Kubota C, Kozai T (1999) Stomatal characteristics and leaf anatomy of potato plantlets cultured in vitro under photoautotrophic and photomixotrophic conditions. In Vitro Cell Dev Biol Plant 35:183–188CrossRefGoogle Scholar

Copyright information

© The Society for In Vitro Biology 2015

Authors and Affiliations

  • A. Barrales-López
    • 1
  • A. Robledo-Paz
    • 1
  • C. Trejo
    • 2
  • E. Espitia-Rangel
    • 3
  • J. L. Rodríguez-De La O
    • 4
  1. 1.Posgrado en Recursos Genéticos y ProductividadColegio de PostgraduadosTexcocoMexico
  2. 2.Posgrado en BotánicaColegio de PostgraduadosTexcocoMexico
  3. 3.Instituto Nacional de Investigaciones ForestalesAgrícolas y Pecuarias (INIFAP)CoatlinchánMexico
  4. 4.Academia de Genética, Departamento de FitotecniaUniversidad Autónoma ChapingoChapingoMexico

Personalised recommendations