In vitro propagation of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] as affected by carbon sources
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This study aimed to establish a protocol for in vitro propagation of two accessions (Ac) of Pfaffia glomerata (Ac 4 and Ac 13) and to evaluate the effect of different carbon sources on the production of 20-hydroxyecdysone (20E) in leaves and roots. For the assessment of axillary shoot proliferation in vitro, nodal segments were inoculated onto Murashige and Skoog (MS) medium supplemented with 2.22 μM 6-benzyladenine and 2.68 μM α-naphthaleneacetic acid and carbon sources (glucose or sucrose) at varying concentrations (0.1, 0.2, or 0.3 M). To assess the in vitro production of 20E, nodal segments were inoculated into Magenta® containers containing MS medium with different carbon sources (glucose, sucrose, or glucose + sucrose at 0.1 or 0.2 M) and placed in plastic bags with bacterial filters. Both experiments were composed of five repetitions for each treatment and analyzed after 30 d of culture. Multiple shoot formations were genotype-dependent when segments were cultivated on a medium supplemented with glucose or sucrose at 0.1 M, yielding 35 and 43 shoots per explant for Ac 4 and 4.4 and 2.8 shoots per explant for Ac 13, respectively. For the 20E content, significant effects were also observed among accessions and carbon sources. Ac 13 had the highest average 20E levels for both roots and leaves. Under the experimental conditions, Ac 4 had more favorable characteristics for large-scale multiplication than Ac 13, and glucose at 0.2 M was the best carbon source for the cultivation of Pfaffia, both for producing multiple shoots and for in vitro 20E production. This is the first report using a combination of auxin and cytokinin to enable effective Pfaffia in vitro axillary shoot proliferation from nodal explants.
KeywordsAxillary proliferation Carbon source Genotype Micropropagation Phytoecdysteroid Organogenesis
This work was supported by the National Council for Scientific and Technological Development (CNPq) [MCT/CNPq 480675/2009-0; PQ 303201/2010-10 to WCO] and a grant from the Minas Gerais State Research Foundation (FAPEMIG) [CAG-APQ-01036-09]. We thank Dr. Roberto Fontes Vieira (National Center for Genetic Resources and Biotechnology–Embrapa/Cenargen, Brasília, DF, Brazil) for providing the Pfaffia glomerata accessions.
- Alves RBN, Bertoni BW, Vieira RF, França SC, Ming LC, Pereira AS (2010) Influência de diferentes meios de cultura sobre o crescimento de Pfaffia glomerata (Spreng.) Pedersen (Amaranthaceae) para conservação in vitro. Rev Bras Plantas Med 12:510–515. doi: 10.1590/S1516-05722010000400016 CrossRefGoogle Scholar
- Figueiredo LS, Teixeira SL, Freitas SP, Vieira IJC, Martins ER (2004) Comportamento de acessos de Pfaffia glomerata (Spreng.) Pedersen (Amaranthaceae) nas condições de Campos dos Goytacazes - RJ. Rev Bras Plantas Med 7:67–72Google Scholar
- Gomes SSL, Saldanha CW, Neves CS, Trevizani M, Raposo NRB, Notini MM, Santos MO, Campos JMS, Otoni WC, Viccini LF (2014) Karyotype, genome size, and in vitro chromosome doubling of Pfaffia glomerata (Spreng.) Pedersen. Plant Cell, Tissue Organ Cult 118:45–56. doi: 10.1007/s11240-014-0460-1 Google Scholar
- Martins CF, Nicoloso FT (2004) Micropropagação de Pfaffia tuberosa (Spreng.) Hicken. Rev Bras Plantas Med 6:53–61Google Scholar
- Nicoloso FT, Erig AC, Martins CF, Russowisk D (2001) Micropropagação do ginseng brasileiro [Pfaffia glomerata (Spreng.) Pedersen]. Rev Bras Plantas Med 3:11–18Google Scholar
- Ruffoni B, Pistelli L, Bertoli A, Pistelli L (2010) Plant cell cultures: bioreactors for industrial production. In: Giardi MT, Rea G, Berra B (eds) Bio-farms for nutraceuticals: functional food and safety control by biosensors. Landes Bioscience and Springer Science + Business Media, pp 203–221Google Scholar
- Saldanha CW, Otoni CG, Rocha DI, Cavatte PC, Detmann KSC, Tanaka FAO, Dias LLC, DaMatta FM, Otoni WC (2014) CO2-enriched atmosphere and supporting material impact the growth, morphophysiology and ultrastructure of in vitro Brazilian-ginseng [Pfaffia glomerata (Spreng.) Pedersen] plantlets. Plant Cell, Tissue Organ Cult 118:87–99. doi: 10.1007/s11240-014-0464-x CrossRefGoogle Scholar
- Sarasan V, Kite GC, Sileshi GW, Stevenson PC (2011) Applications of phytochemical and in vitro techniques for reducing over-harvesting of medicinal and pesticidal plants and generation income for the rural poor. Plant Cell Rep 30:1163–1172. doi: 10.1007/s00299-011-1047-5 PubMedCrossRefGoogle Scholar
- Souza VC, Lorenzi H (2005) Botânica Sistemática: guia ilustrado para identificação das famílias de Angiospermas da flora brasileira, baseado em APG II. Instituto Plantarum, Nova Odessa, pp 220–223Google Scholar
- Vigo CLS, Narita E, Milaneze-Gutierre MA, Marques LC (2004) Caracterização farmacognóstica comparativa de Pfaffia glomerata (Spreng.) Pedersen Hebanthe paniculata Martius—Amaranthaceae. Rev Bras Plantas Med 6:7–19Google Scholar