Expression and epigenetic profile of protoplast cultures (Cucumis sativus L.)
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Cellular dedifferentiation is a process in which cells change from a given differentiated state into a transient, stem cell-like state and reenter into the cell cycle (Grafi 2004). In plants, cellular dedifferentiation is exemplified by protoplastization. Here, the cell wall of differentiated leaf cells is degraded by given enzymes that finish by protoplasts’ formation (Zhao et al.2001). The protoplasts assume a unique transient state with features resembling animal stem cells (Grafi et al.2011). The global chromatin decondensation is characterized for plant protoplasts (Zhao et al.2001; Tessadori et al.2007; Ondřej et al.2009b, 2010) that is also a distinguishing feature of animal stem cells. Various stimuli, that include phytohormones like auxins and cytokinins, induce protoplasts to reenter the cell cycle and proliferate. The reprogrammed cells could form calli, which under appropriate conditions can form shoots and roots, somatic embryos, and eventually fertile plant...
KeywordsProtoplast Isolation Protoplast Culture Cell Wall Modification Cell Wall Regeneration Chromatin Decondensation
This work was supported by the IGA UP Agency Grants PrF/2013/003 and PrF/2014/001.
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