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An efficient method for regeneration of lavandin (Lavandula x intermedia cv. 'Grosso')

  • Lauren A. E. Erland
  • Soheil S. Mahmoud
Protocols/Methods

Abstract

An efficient protocol for the regeneration of lavandin (Lavandula x intermedia cv. 'Grosso') is reported. Thiadazuron (9 μM), a plant growth-modulating phenylurea, was used to induce callus formation and shoot initiation from cultured leaf explants. Newly emerged shoots were maintained on media containing 0.05 μM naphthaleneacetic acid to allow maturation, and then transferred to media containing 2.9 μM indole-3-acetic acid to allow root formation. The phenolic control agents polyvinylpyrrolidone (PVP), ascorbic acid, 2-aminoindane-2-phosphonic acid, and activated charcoal were tested for their ability to prevent shoot browning and death in culture. All agents except PVP were found to be effective, with ascorbic acid being most consistent in promoting development of healthy mature shoots. The effect of light type (red light vs. white light) and culture medium composition (full- and half-strength Murashige and Skoog or Llyod and McCown’s woody plant medium (WPM)) on rooting efficiency was also evaluated. Cultures on half-strength WPM in white light were found to have the highest rooting efficiency. Additionally, application of the polyamines putrescine, spermine, and spermidine were tested for their effect on rooting. While rooting efficiency was not improved with any of the treatments, spermine and spermidine were found to have an inhibitory effect at concentrations greater than 10 μM.

Keywords

Lavandin L. x intermedia Regeneration Organogenesis Rooting efficiency 

Notes

Acknowledgments

This work was supported through grants or in-kind contributions to Soheil Mahmoud by UBC, Natural Sciences and Engineering Research Council of Canada, Canada Foundation for Innovation, and industrial partners including Okanagan Lavender Herb Farm (Kelowna, B.C.) and Downderry Nursery Ltd (Tonbridge, UK). Financial support was also provided by the Investment Agriculture Foundation of B.C. through programs it delivers on behalf of Agriculture and Agri-Food Canada and the B.C. Ministry of Agriculture. We would like to thank Dr. Susan Murch for generously providing the AIP for this study and Broc Glover for his assistance in manuscript proof-reading.

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Copyright information

© The Society for In Vitro Biology 2014

Authors and Affiliations

  1. 1.Department of BiologyUniversity of British ColumbiaKelownaCanada

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