An improved protocol for carrot haploid and doubled haploid plant production using induced parthenogenesis and ovule excision in vitro

Table 4. Mean percentage of ovules, developing embryos, or calli in vitro depending on medium composition. Unpollinated controls (UU and US) are excluded

Year	Symbol	Medium		No. of plated ovules	Mean percentage of ovules developing
Year	Symbol	Minerals, vitamins	Supplement	No. of plated ovules	Embryos		Calli
2008	H	1/2 MS	0.06 μM IAA	7,160	0.15	a	0.07	a
2008	O	1/2 MS	4.16 mM Edamin K	6,182	0.48	a	0.24	a
2009	H	1/2 MS	0.06 μM IAA	9,695	0.36	a	0.15	bc
	HP	1/2 MS	0.06 μM IAA 1 mM putrescine	8,231	0.46	a	0.47	a
	SHI	MS	0.57 μM IAA 1.44 μM GA₃ 4.60 μM KIN	8,520	0.49	a	0.22	b
	SHP	MS	0.57 μM IAA 1.44 μM GA₃ 4.60 μM KIN 1 mM putrescine	8,880	0.32	a	0.03	c
2010	H	1/2 MS	0.06 μM IAA	9,020	1.70	ab	0.01	c
	HT	1/2 MS	0.06 μM IAA 9.08 μM TDZ	7,260	2.14	a	1.69	a
	SHI	MS	0.57 μM IAA 1.44 μM GA₃ 4.60 μM KIN	7,840	1.42	ab	0.32	b
	SHT	MS	0.57 μM IAA 1.44 μM GA₃ 4.60 μM KIN 9.08 μM TDZ	8,760	1.07	b	0.53	b

Means followed by the same letters in a column within each year do not differ significantly according to Fisher’s LSD test at p = 0.05