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Suppression of ethylene levels promotes morphogenesis in pepper (Capsicum annuum L.)

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Abstract

Ethylene and polyamines (PAs) are two phytohormones that play important roles during in vitro morphogenesis of several plant species. The interaction between ethylene and PAs has been of interest because both have S-adenosylmethionine as a precursor. To study the influence of ethylene and PAs on in vitro morphogenesis of an ornamental pepper, we added an ethylene scavenger, PAs, a PA inhibitor, and compounds that affect ethylene biosynthesis and activity to the regeneration medium. Regeneration frequencies increased in response to treatment with ethylene inhibitors (aminoethoxyvinylglycine and silver thiosulfate) and an ethylene scavenger (mercury perchlorate). Treatment with the ethylene precursor 1-aminocyclopropane-1-carboxylic acid reduced the regeneration frequency, increased callus formation, and increased ethylene levels; similar results were obtained in response to treatment with the PA inhibitor methylglyoxal-bis(guanylhydrazone). By contrast, treatment with PAs (particularly spermidine and spermine) decreased ethylene levels, increased the regeneration frequency, and increased shoot bud formation. These results suggest a coordinated regulation of ethylene and polyamines because the suppression of ethylene levels using ethylene inhibitors, polyamines, or mercury perchlorate increased the in vitro regeneration frequency and morphogenic responses of Capsicum annuum L.

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Acknowledgments

The authors thank the Brazilian sponsoring agencies CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil), FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais), and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior) for financial support.

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Authors and Affiliations

  1. Laboratório de Cultura de Tecidos Vegetais (LCTII), Departamento de Biologia Vegetal/BIOAGRO, Universidade Federal de Viçosa, Av. P.H. Rolfs s/n, Campus Universitário, 36570-000, Viçosa, MG, Brazil

    Diego Silva Batista, Leonardo Lucas Carnevalli Dias & Wagner Campos Otoni

  2. Laboratório de Biologia Celular (BIOCEL), Instituto de Ciências Biológicas, Departamento de Botânica, Universidade de São Paulo (USP), 11461, São Paulo, SP, Brazil

    Amanda Ferreira Macedo & Eny Iochevet Segal Floh

  3. Centro de Ciências Agrárias, Universidade Federal da Paraíba, BR 079, Km 12, Campus II, 58397-000, Areia, PB, Brazil

    Mailson Monteiro do Rêgo & Elizanilda Ramalho do Rêgo

  4. Departamento de Fitotecnia, Universidade Federal de Viçosa, Av. P.H. Rolfs s/n, Campus Universitário, 36570-000, Viçosa, MG, Brazil

    Fernando Luiz Finger

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  1. Diego Silva Batista
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  2. Leonardo Lucas Carnevalli Dias
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  8. Wagner Campos Otoni
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Correspondence to Wagner Campos Otoni.

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Batista, D.S., Dias, L.L.C., Macedo, A.F. et al. Suppression of ethylene levels promotes morphogenesis in pepper (Capsicum annuum L.). In Vitro Cell.Dev.Biol.-Plant 49, 759–764 (2013). https://doi.org/10.1007/s11627-013-9559-z

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