Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light

Plant Tissue Culture

Abstract

An efficient root induction system has been established for in vitro-regenerated Jatropha curcas L. shoots. Callus formation on shoots transferred to auxin containing medium was found to be a prominent and recurrent problem for rooting of in vitro-cultivated J. curcas. In particular, the type of auxins and cytokinins applied in the culture media were shown to strongly influence the severity of callus formation. Shoots cultivated on meta-methoxytopolin riboside (MemTR) were free of callus and produced elongated stems and well-developed leaves in comparison to the cytokinins benzyl adenine, zeatin, and thidiazuron. Subsequent root induction experiments were performed with shoots precultured on MemTR-containing medium. Shoots were excised and transferred to Murashige and Skoog (MS) medium supplemented with different concentrations of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), and α-naphtaleneacetic acid (NAA). The induction of excessive callus formation was avoided only on IBA-containing medium. The optimum rooting medium with good root induction (35%) and 1.2 roots per shoot contained half-strength MS salts supplemented with 2.5 μM IBA. The same medium supplemented with 0.25% (w/v) activated charcoal produced 46% rooted shoots. Further improvement of rooting was obtained by transferring in vitro grown shoots to woody plant medium containing phloroglucinol (PG). In the presence of 2.5 μM IBA and 238 μM PG, 83% of the shoots rooted with on average 3.1 roots per shoot. We also analyzed the impact of light quality on the rooting capacity of Jatropha in vitro grown shoots. In general, light-emitting diodes (LEDs) light sources were less efficient for root induction. Red LED light provided the most favorable growth conditions, inducing a rooting response in 65% of the shoots, which produced on average 5.5 roots per shoot. These results indicate that adventitious rooting in J. curcas is under control of photoreceptors and that optimal rooting requires fine-tuning of the salt concentration, auxin, and cytokinin balance and application of synergistic compounds.

Keywords

In vitro root induction Jatropha curcas Light-emitting diodes (LEDs) Phloroglucinol 

References

  1. Bairu MW, Stirk WA, Van Staden J (2009) Factors contributing to in vitro shoot-tip necrosis and their physiological interactions. Plant Cell Tiss Organ Cult 98:239–248CrossRefGoogle Scholar
  2. Baker CM, Wetzstein HY (1994) Influence of auxin type and concentration on peanut somatic embryogenesis. Plant Cell Tiss Organ Cult 36:361–368CrossRefGoogle Scholar
  3. Baque MA, Hahn EJ, Paek KY (2010) Induction mechanism of adventitious root from leaf explants of Morinda citrifolia as affected by auxin and light quality. In Vitro Cell Dev Plant 46:71–80Google Scholar
  4. Bhatt B, Tomar Y (2010) Effects of IBA on rooting performance of Citrus auriantifolia Swingle (Kagzi-lime) in different growing conditions. Nat Sci 8:8–11Google Scholar
  5. Datta MM, Mukherjee P, Ghosh B, Jha TB (2007) In vitro clonal propagation of biodiesel plant (Jatropha curcas L.). Curr Sci 93:1438–1442Google Scholar
  6. Davis T, Haissig B (1994) Biology of adventitious root formation. Plenum Press, New YorkGoogle Scholar
  7. De Klerk GJ (2002) Rooting of microcuttings: theory and practice. In Vitro Cell Dev Plant 38:415–422CrossRefGoogle Scholar
  8. De Klerk GJ, Guan HY, Huisman P, Marinova S (2011) Effects of phenolic compounds on adventitious root formation and oxidative decarboxylation of applied indoleacetic acid in Malus ‘Jork 9’. Plant Growth Regul 63:175–185CrossRefGoogle Scholar
  9. Deore AC, Johnson TS (2008) High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L.: an important biodiesel plant. Plant Biotechnol Rep 2:7–11CrossRefGoogle Scholar
  10. Devappa R, Makkar H, Becker K (2011) Jatropha diterpenes: a review. J Am Oil Chem Soc 88:301–322CrossRefGoogle Scholar
  11. Dubranszki J, da Silva JAT (2010) Micropropagation of apple—a review. Biotechnol Adv 28:462–488CrossRefGoogle Scholar
  12. Gabryszewska E, Rudnicki RM (1997) The effects of light quality on the growth and development of shoots and roots of Ficus benjamina in vitro. Acta Hortic 418:163–167Google Scholar
  13. George EF (2008) Plant propagation by tissue culture, 3rd edition, vol 1. The background. In: George EF, Hall MA, De Klerk G-J (eds) Plant tissue culture procedure—background. Springer, Dordrecht, pp 1–28Google Scholar
  14. Hammatt N (1994) Promotion by phloroglucinol of adventitious root formation in micropropagated shoots of adult wild cherry (Prunus avium L.). Plant Growth Regul 14:127–132CrossRefGoogle Scholar
  15. Hammatt N, Grant NJ (1997) Micropropagation of mature British wild cherry. Plant Cell Tiss Organ Cult 47:103–110CrossRefGoogle Scholar
  16. Iacona C, Muleo R (2010) Light quality affects in vitro adventitious rooting and ex vitro performance of cherry rootstock Colt. Sci Hortic 125:630–636CrossRefGoogle Scholar
  17. Igbinosa OO, Igbinosa EO, Aiyegoro OA (2009) Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn). Afr J Pharm Pharmaco 3:58–62Google Scholar
  18. James DJ, Thurbon IJ (1981) Shoot and root initiation in vitro in the apple rootstock M9 and the promotive effects of phloroglucinol. J Hortic Sci 56:15–20Google Scholar
  19. Jones OP (1976) Effect of phloridzin and phloroglucinol on apple shoots. Nature 262:392–393CrossRefGoogle Scholar
  20. Jones OP, Hatfield SGS (1976) Root initiation in apple shoots cultured in vitro with auxins and phenolic compounds. J Hortic Sci 51:495–500Google Scholar
  21. Kalimuthu K, Paulsamy S, Senthilkumar R, Sathya M (2007) In vitro propagation of the biodiesel plant Jatropha curcas. Plant Tiss Cult Biotechnol 17:137–147Google Scholar
  22. Kumar N, Vijay Anand K, Reddy M (2011) Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants. J Plant Biochem Biot 20:125–133CrossRefGoogle Scholar
  23. Kumar S, Kumaria S, Tandon P (2010) Efficient in vitro plant regeneration protocol from leaf explant of Jatropha curcas L. - a promising biofuel plant. J Plant Biochem Biot 19:275–277Google Scholar
  24. Li M, Li H, Jiang H, Pan X, Wu G (2008) Establishment of an Agrobacteriuim-mediated cotyledon disc transformation method for Jatropha curcas. Plant Cell Tiss Organ Cult 92:173–181CrossRefGoogle Scholar
  25. Lloyd G, McCown B (1981) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Combined Proc Int Plant Propagation Soc 30:421–426Google Scholar
  26. Mazumdar P, Basu A, Paul A, Mahanta C, Sahoo L (2010) Age and orientation of the cotyledonary leaf explants determine the efficiency of de novo plant regeneration and Agrobacterium tumefaciens-mediated transformation in Jatropha curcas L. S Afr J Bot 76:337–344CrossRefGoogle Scholar
  27. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  28. Openshaw K (2000) A review of Jatropha curcas: an oil plant of unfulfilled promise. Biomass Bioenerg 19:1–15CrossRefGoogle Scholar
  29. Oskoueian E, Abdullah N, Ahmad S, Saad WZ, Omar AR, Ho YW (2011) Bioactive compounds and biological activities of Jatropha curcas L. kernel meal extract. Int J Mol Sci 12:5955–5970PubMedCrossRefGoogle Scholar
  30. Ozel C, Khawar K, Mirici S, Arslan O, Sebahattin O (2006) Induction of ex vitro adventitious roots on softwood cuttings of Centaurea tchihatcheffii tchihatcheffii Fisch. et. Mey using indole-3-butyric acid and naphthalene acetic acid. Int J Agric Biol 1:66–69Google Scholar
  31. Poudel PR, Kataoka I, Mochioka R (2008) Effect of red and blue light emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss Organ Cult 92:147–153CrossRefGoogle Scholar
  32. Rajore S, Batra A (2005) Efficient plant regeneration via shoot tip explant in Jatropha curcas L. J Plant Biochem Biot 14:73–75CrossRefGoogle Scholar
  33. Reddy MP, Pamidimarri DVNS (2010) Desert plants biology and biotechnology. In: Ramawat KG (ed) Biology and biotechnological advances in Jatropha curcas—a biodiesel plant desert plants. Springer, Berlin, pp 57–71Google Scholar
  34. Reubens B, Achten WMJ, Maes WH, Danjon F, Aerts R, Poesen J, Muys B (2011) More than biofuel? Jatropha curcas root system symmetry and potential for soil erosion control. J Arid Environ 75:201–205CrossRefGoogle Scholar
  35. Rout GR, Samantaray S, Das P (2000) In vitro manipulation and propagation of medicinal plants. Biotechnol Adv 18:91–120PubMedCrossRefGoogle Scholar
  36. Severino LS, Lima RLS, Lucena AMA, Freire MAO, Sampaio LR, Veras RP, Medeiros KAAL, Sofiatti V, Arriel NHC (2011) Propagation by stem cuttings and root system structure of Jatropha curcas. Biomass Bioenerg 35:3160–3166CrossRefGoogle Scholar
  37. Shrivastava S, Banerjee M (2008) In vitro clonal propagation of physic nut (Jatropha curcas L.): influence of additives. Int J Integ Biol 3:73–79Google Scholar
  38. Sujatha M, Makkar HPS, Becker K (2005) Shoot bud proliferation from axillary nodes and leaf sections of non-toxic Jatropha curcas L. Plant Growth Regul 47:83–90CrossRefGoogle Scholar
  39. Sujatha M, Reddy TP, Mahasi MJ (2008) Role of biotechnological interventions in the improvement of castor (Ricinus communis L.) and Jatropha curcas L. Biotechnol Adv 26:424–435PubMedCrossRefGoogle Scholar
  40. Tennessen D, Singsaas E, Sharkey T (1994) Light-emitting diodes as a light source for photosynthesis research. Photosynthesis Res 39:85–92CrossRefGoogle Scholar
  41. Wagenknecht AC, Burris RH (1950) Indoleacetic acid inactivating enzymes from bean roots and pea seedlings. Arch Biochem 25:30–53PubMedGoogle Scholar

Copyright information

© The Society for In Vitro Biology 2013

Authors and Affiliations

  1. 1.Department of Biology, Faculty of Science and MathematicsSultan Idris Education UniversityTanjong MalimMalaysia
  2. 2.Department of Plant Production, Faculty of Bioscience EngineeringGhent UniversityGhentBelgium

Personalised recommendations