In vitro propagation of the Damask rose (Rosa damascena Mill.)

  • Haleh Mahmoudi Noodezh
  • Ahmad Moieni
  • Amin Baghizadeh
Plant Tissue Culture

Abstract

Roses are an important commercial crop available in a wide range of varieties in international markets. Due to its economic value, this study aimed to establish a new and reproducible protocol for the in vitro propagation of Rosa damascena Mill. We developed an efficient and cost-effective method for rapid and high-quality shoot multiplication and in vitro rooting of Damask rose using nodal explants. For each stage of the micropropagation procedure (i.e., explant establishment, shoot multiplication and growth, and rooting), different media and combinations of plant growth regulators were utilized. A new culture medium, termed A19, resulted in significant improvements to shoot proliferation and root induction for this rose cultivar. For optimal explant establishment, shoot growth, and proliferation, a modified Murashige and Skoog medium with higher levels of nitrates, calcium, and iron plus supplementation with 4 mg/l 6-benzylaminopurine and 0.25 mg/l indole-3-acetic acid was utilized. To increase shoot length, 75 d after culture initiation (including two subcultures), shoots were transferred to the same medium additionally supplemented with 0.2 mg/l gibberellic acid. This resulted in vigorous shoot growth, with longer shoots and a greater number of shoots per explant. Shoots were then separated and transferred to various root induction medium for 30 d. The results clearly showed that a liquid ½A19 medium-A (i.e., with half-strength macroelements) supplemented with 0.1 mg/l indole-3-butyric-acid was the most successful medium for in vitro rooting in this cultivar. Shoots were cultured in this medium for 7 d in the dark, before transfer to liquid ½A19 medium-A without hormone supplementation under a 16-h photoperiod. This modified protocol resulted in significant improvement in shoot regeneration and proliferation and obtained stronger shoots over a period of about 20 wk.

Keywords

Rosa damascena Mill. Damask rose Micropropagation In vitro rooting 

References

  1. Achuthan CR, Babu BH, Padikkala J (2003) Antioxidant and hepatoprotective effects of Rosa damascena. Pharm Biol 41:357–361CrossRefGoogle Scholar
  2. Alderson PG, McKinless J, Rice RD (1988) Rooting of cultured rose shoots. Acta Hort 226:175–182Google Scholar
  3. Badzian T, Hennen GR, Fotyma-Kern J (1991) In vitro rooting of clonal propagated miniature rose cultivars. Acta Hort 289:329–330Google Scholar
  4. Basim E, Basim H (2003) Antibacterial activity of Rosa damascene essential oil. Fitoterapia 74:394–396PubMedCrossRefGoogle Scholar
  5. Bordbar L (2005) Optimization of shoots proliferation for Damask rose micropropagation. MSc Thesis pp 1−80Google Scholar
  6. Bressan PH, Kim YJ, Hyndman SE, Hasegawa PM, Bressan RA (1982) Factors affecting in vitro propagation of rose. J Am Soc Horti Sci 107:979–990Google Scholar
  7. Curir P, Damiano C, Cosmi T (1986) In vitro propagation of some rose cultivars. Acta Hort 189:221–224Google Scholar
  8. Dohare SR, Shafi M, Kaicker US (1991) Micropropagation in roses. Acta Hort 289:107–108Google Scholar
  9. Douglas GC, Rutledge CB, Casey AD, Richardson DHS (1989) Micropropagation of floribunda, ground cover and miniature roses. Plant Cell Tiss Organ Cult 19:55–64CrossRefGoogle Scholar
  10. Hasegawa PM (1980) Factors affecting shoot and root initiation from cultured rose shoot tips. J Am Soc Hortic Sci 105:216–220Google Scholar
  11. Horn WAH (1992) Micropropagation of rose (Rosa L.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 20: high-tech and micropropagation IV. SpringerVerlag, Germany, pp 320–342Google Scholar
  12. Ibrahim AI (1994) Effect of gelling agent and activated charcoal on the growth and development of cordyline terminalis cultured in vitro. Proceedings of the first conference of ornamental horticulture 1:55–67Google Scholar
  13. Jabbarzadeh Z, Khosh-Khui M (2005) Factors affecting tissue culture of Damask rose (Rosa damascena Mill.). Sci Hortic 105:475–482CrossRefGoogle Scholar
  14. Khosh-Khui M, Sink KC (1982) Rooting-enhancement of Rosa hybrida for tissue culture propagation. Sci Hortic 17:371–376CrossRefGoogle Scholar
  15. Kirichenko EB, Kuz-mina TA, Kataeva NV (1991) Factors in optimizing the multiplication of ornamental and essential oil roses in vitro. Bull Gl Bot Sada 159:61–67Google Scholar
  16. Kumar A, Sood A, Palni UT, Gupta AK, Palni LMS (2001) Micropropagation of Rosa damascena Mill. from mature bushes using thidiazuron. J Hortic Sci Biotechnol 76:30–34Google Scholar
  17. Mahmood N, Piacente S, Pizza C, Burke A, Khan A, Hay A (1996) The anti-HIV activity and mechanisms of action of pure compounds isolated from Rosa damascene. Biochem Biophys Res Commun 229:73–79PubMedCrossRefGoogle Scholar
  18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  19. Ozkan G, Sagdic O, Baydar NG, Baydar H (2004) Antioxidant and antibacterial activities of Rosa damascena flower extracts. Food Sci Technol Int 10:277–281CrossRefGoogle Scholar
  20. Pati PK, Sharma M, Sood A, Ahuja PS (2005) Micropropagation of Rosa damascena and R. bourboniana in liquid cultures. In: Hvoslef-Eide AK, Preil W (eds) Liquid systems for in vitro plant propagation. SpringerVerlag, Springer e-book, pp 373–385CrossRefGoogle Scholar
  21. Pati PK, Rath SP, Sharma M, Sood A, Ahuja PS (2006) In vitro propagation of rose—a review. Biotechnol Adv 24:94–114PubMedCrossRefGoogle Scholar
  22. Podwyszynska M, Olszewski T (1995) Influence of gelling agents on shoot multiplication and the uptake of macroelements by in vitro culture of rose, cordyline and homalomena. Sci Hortic 64:77–84CrossRefGoogle Scholar
  23. Rout GR, Debata BK, Das P (1989) In vitro mass-scale propagation of Rosa hybrida cv. Landora. Curr Sci 58:876–878Google Scholar
  24. Sauer A, Walther F, Preil W (1985) Different suitability for in vitro propagation of rose cultivars. Gartenbauwissenschaft 50:133–138Google Scholar
  25. Valles M, Boxus P (1987a) Micropropagation of several Rosa hybrida L. cultivars. Acta Hort 212:611–618Google Scholar
  26. Valles M, Boxus P (1987b) Regeneration from Rosa callus. Acta Hort 212:691–696Google Scholar
  27. Van der Salm TPM, Van der Toorn CJG, Hänisch ten Cate CH, Dubois LAM, De Vries DP, Dons HJM (1994) Importance of the iron chelate formula for micropropagation of Rosa hybrida L. ‘Moneyway’. Plant Cell Tiss Organ Cult 37:73–77CrossRefGoogle Scholar
  28. Wulster G, Sacalis J (1980) Effects of auxins and cytokinins on ethylene evolution and growth of rose callus tissue in sealed vessels. HortSci 15:736–737Google Scholar

Copyright information

© The Society for In Vitro Biology 2012

Authors and Affiliations

  • Haleh Mahmoudi Noodezh
    • 1
  • Ahmad Moieni
    • 1
  • Amin Baghizadeh
    • 2
  1. 1.Department of Plant Breeding and Biotechnology, Faculty of AgricultureTarbiat Modares UniversityTehranIran
  2. 2.Department of BiotechnologyInternational Center for Science, High Technology and Environmental SciencesKermanIran

Personalised recommendations