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Food-grade sugar can promote differentiation in melon (Cucumis melo L.) tissue culture

In Vitro Cellular & Developmental Biology - Plant volume 48, pages 600–608 (2012)Cite this article

Abstract

The objective of the present study was to investigate the origin of discrepancy between experimental results in in vitro culture of Turkish melon (Cucumis melo L.) cultivars, conducted by the same individual using the same protocol and same seed batches in two different laboratories. The difference in the sucrose source was found to be the major reason for the deviation in results between the two laboratories. The percentage of regenerating explants and the number of bud-like protuberances and/or shoots were significantly greater when a food-grade Turkish sucrose was used in the medium compared with analytical-grade sucrose. Media formulated with the food-grade sucrose regenerated 37 and 67 % more explants and bud-like protuberances and/or shoots per explant, respectively, than media containing analytical-grade sucrose. No meaningful differences were found in added elements or anions between the sucrose sources or by liquid chromatography/mass spectroscopy. The only significant chemical difference observed between the sucrose samples was the presence of melanoidins (Maillard reaction products) in the food-grade sucrose. The melanoidins were of high molecular weight (>3,000 Da determined by ultrafiltration), with characteristic ultraviolet–visible spectra and in vitro antioxidant activity. Melanoidin-containing sucrose can be differentiated by color and spectroscopy.

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References

  • Amutha S, Muruganantham M, Ananthakrishnan G, Yablonsky S, Singer S, Gaba V (2009) Improved shoot regeneration due to prolonged seed storage. Sci Hort 119:117–119

    Article  CAS  Google Scholar 

  • Bartlett MS (1936) The square root transformation in analysis of variance. J Royal Statist Soc Suppl 3:68–78

    Article  Google Scholar 

  • Bartlett MS (1947) The use of transformations. Biometrics 3:39–52

    PubMed  Article  CAS  Google Scholar 

  • Borrelli RC, Fogliano V, Monti SM, Ames JM (2002) Characterization of melanoidins from a glucose–glycine model system. Eur Food Res Technol 215:210–215

    Article  CAS  Google Scholar 

  • Bosetto M, Arfaioli P, Ugolini FC, Degl’ Innocenti A, Agnelli E, Corti G (2006) Synthesis and characterization of Maillard compounds formed under sterile conditions on sand and silt-sized mineral substrates. Comm Soil Science Plant Anal 37:1043–1058

    Article  CAS  Google Scholar 

  • Brands CMJ, Wedzicha BL, van Boekel MAJS (2002) Quantification of melanoidin concentration in sugar–casein systems. J Agric Food Chem 50:1178–1183

    PubMed  Article  CAS  Google Scholar 

  • Brudzynski K, Miotto D (2011) The recognition of high molecular weight melanoidins as the main components responsible for radical-scavenging capacity of unheated and heat-treated Canadian honeys. Food Chem 125:570–575

    Article  CAS  Google Scholar 

  • Canellas LP, Spaccini R, Piccolo A, Dobbss LB, Okorokova-Facanha AL, Santos GD, Olivares FL, Facanha AR (2009) Relationships between chemical characteristics and root growth promotion of humic acids isolated from Brazilian oxisols. Soil Sci 174:611–620

    Article  CAS  Google Scholar 

  • Compton ME (1994) Statistical methods suitable for analysis of plant tissue culture data. Plant Cell Tiss Org Cult 37:217–242

    Google Scholar 

  • Çürük S, Çetiner S, Gaba V (2002) In vitro regeneration of some Turkish melon (Cucumis melo L.) cultivars. Biotechnol Biotechnol Equip 16:39–46

    Google Scholar 

  • Dahleen LS, Bregitzer P (2002) An improved media system for high regeneration rates from barley immature embryo-derived callus cultures of commercial cultivars. Crop Sci 42:934–938

    Article  Google Scholar 

  • Debeaujon I, Branchard M (1992) Introduction of somatic embryogenesis and caulogenesis from cotyledon and leaf protoplast-derived colonies of melon (Cucumis melo L.). Plant Cell Rep 12:37–40

    Article  Google Scholar 

  • El-Bakry AA (2002) Effect of genotype, growth regulators, carbon source and pH on shoot induction and plant regeneration in tomato. In Vitro Cell Dev Biol Plant 38:501–507

    Article  CAS  Google Scholar 

  • Elena A, Diane L, Eva B, Marta F, Roberto B, Zamarreno AM, Garcia-Mina JM (2009) The root application of a purified leonardite humic acid modifies the transcriptional regulation of the main physiological root responses to Fe deficiency in Fe-sufficient cucumber plants. Plant Physiol Biochem 47:215–223

    PubMed  Article  Google Scholar 

  • Facanha AR, Facanha ALO, Olivares FL, Guridi F, Santos GD, Velloso ACX, Rumjanek VM, Brasil F, Schripsema J, Braz R, de Oliveira MA, Canellas LP (2002) Humic acids bioactivity: effects on root development and on the plasma membrane proton pump. Pesquisa Agropecuaria Brasileira 37:1301–1310

    Article  Google Scholar 

  • Fang G, Grumet R (1990) Agrobacterium tumefaciens mediated transformation and regeneration of muskmelon plants. Plant Cell Rep 9:160–164

    Article  CAS  Google Scholar 

  • Gaba V, Schlarman E, Elman C, Sagee O, Watad AA, Gray DJ (1999) In vitro studies on the anatomy and morphology of bud regeneration in melon cotyledons. In Vitro Cell Dev Biol Plant 35:1–7

    Google Scholar 

  • Grandison AS, Lewis MJ (1996) Separation processes in the food and biotechnology industries: principles and applications. Woodhead Publishing, Cambridge

    Book  Google Scholar 

  • Huang D, Ou B, Prior RL (2005) The chemistry behind antioxidant assays. J Agric Food Chem 53:1841–1856

    PubMed  Article  CAS  Google Scholar 

  • Ikan R, Ioselis P, Rubinsztain Y, Aizenshtat Z, Frenkel M, Peters KE (1994) Pyrolysis of natural and synthetic humic substances. J Thermal Anal 42:31–40

    Article  CAS  Google Scholar 

  • Ikan R, Ioselis P, Rubinsztain Y, Aizenshtat Z, Pugmire R, Anderson LL (1986) Carbohydrate origin of humic substances. Naturwissensch 73:150–151

    Article  CAS  Google Scholar 

  • Ishiwatari R, Morinaga S, Yamamoto S, Machihara T, Rubinsztain Y, Ioselis P, Aizenshtat Z, Ikan R (1986) A study of formation mechanism of sedimentary humic substances. 1. Characterization of synthetic humic substances (melanoidins) by alkaline potassium permaganate oxidation. Organic Geochem 9:11–23

    Article  CAS  Google Scholar 

  • Lou H, Kako S (1994) Somatic embryogenesis and plant regeneration in cucumber. Hortscience 29:906–909

    Google Scholar 

  • Maillard LC (1912) Formation of humus and combustible minerals without the influence of atmospheric oxygen, microorganisms, high temperatures or high pressure. C R Acad Sci 154:66–68

    CAS  Google Scholar 

  • Maillard LC (1917) General reaction between amino acids and sugars: the biological consequences. C R Soc Biol 72:599–601

    Google Scholar 

  • Meurer CA, Dinkins RD, Redmond CT, McAllister KP, Tucker DT, Walker DR, Parrott WA, Trick HN, Essig JS, Frantz HM, Finer JJ, Collins GB (2001) Embryogenic response of multiple soybean [Glycine max (L.) Merr.] cultivars across three locations. In Vitro Cell Dev Biol Plant 37:62–67

    Google Scholar 

  • Morales FJ (2002) Application of capillary zone electrophoresis to the study of food and food-model melanoidins. Food Chem 76:363–369

    Article  CAS  Google Scholar 

  • Morales FJ, Jimenez-Perez S (2004) Peroxyl radical scavenging activity of melanoidins in aqueous systems. Eur Food Res Technol 218:515–520

    Article  CAS  Google Scholar 

  • Moreno V, Garcia-Sogo M, Granell I, Garcia-Sogo B, Roig LA (1985) Plant regeneration from calli of melon (Cucumis melo L. cv. ‘Amarillo Oro’). Plant Cell Tiss Org Cult 5:139–146

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assay with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Muscolo A, Panuccio MR, Sidari M, Sessi E, Nardi S (2002) Alteration of amino acid metabolism by humic substances during germination of Pinus laricio seeds. Seed Sci Tech 30:205–210

    Google Scholar 

  • Nardi S, Panuccio MR, Abenavoli MR, Muscolo A (1994) Auxin-like effect of humic substances extracted from feces of Allolobophora caliginosa and A. rosea. Soil Biol Biochem 26:1341–1346

    Article  CAS  Google Scholar 

  • Niedz RP, Smith SS, Dunbar KB, Stephens CT, Murakishi HH (1989) Factors influencing shoot regeneration from cotyledonary explants of Cucumis melo. Plant Cell Tiss Org Cult 18:313–319

    Article  Google Scholar 

  • Pinton R, Varanini Z, Vizzotto G, Maggioni A (1992) Soil humic substances affect transport-properties of tonoplast vesicles isolated from oat roots. Plant Soil 142:203–210

    Article  CAS  Google Scholar 

  • Poonsapaya P, Nabors MV, Wright K, Vajrabhaya M (1989) A comparison of methods for callus culture and plant regeneration of RD25 rice (Oryza sativa L.) in two laboratories. Plant Cell Tiss Org Cult 16:175–186

    Google Scholar 

  • Rufian-Henares JA, de la Cueva SP (2009) Antimicrobial activity of coffee melanoidins—a study of their metal-chelating properties. J Agric Food Chem 57:432–438

    PubMed  Article  CAS  Google Scholar 

  • Rufian-Henares JA, Morales FJ (2007) Antimicrobial activity of melanoidins. J Food Qual 30:160–168

    Article  CAS  Google Scholar 

  • Ruiz-Roca B, Navarro MP, Seiquer I (2008) Antioxidant properties and metal chelating activity of glucose-lysine heated mixtures: relationships with mineral absorption across Caco-2 cell monolayers. J Agric Food Chem 56:9056–9063

    PubMed  Article  CAS  Google Scholar 

  • Trevisan S, Pizzeghello D, Ruperti B, Francioso O, Sassi A, Palme K, Quaggiotti S, Nardi S (2011) Humic substances induce lateral root formation and expression of the early auxin-responsive IAA19 gene and DR5 synthetic element in Arabidopsis. Plant Biol 12:604–614

    Google Scholar 

  • Tyagi RK, Agrawal A, Mahalakshmi C, Hussain Z, Tyagi H (2007) Low-cost media for in vitro conservation of turmeric (Curcuma longa L.) and genetic stability assessment using RAPD markers. In Vitro Cell Dev Biol Plant 43:51–58

    Article  CAS  Google Scholar 

  • Vallés MP, Lasa JM (1994) Agrobacterium-mediated transformation of commercial melon (Cucumis melo L., cv. Amarillo Oro). Plant Cell Rep 13:145–148

    Article  Google Scholar 

  • Vignoli JA, Bassoli DG, Benassi MT (2011) Antioxidant activity, polyphenols, caffeine and melanoidins in soluble coffee: the influence of processing conditions and raw material. Food Chem 124:863–868

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, No. 510/11. This work was supported by a fellowship and grant to S. Çürük from the Scientific and Technical Research Council of Turkey (TUBITAK/BAYG) and to VG by the Chief Scientist of the Ministry of Agriculture, Israel. We are grateful to Dr. B. Steinitz for review of an early version of the manuscript, and to Dr. A.A. Schaffer for helpful comments and assistance.

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Authors and Affiliations

  1. Faculty of Agriculture, Department of Horticulture, Mustafa Kemal University, 31034, Antakya, Hatay, Turkey

    Sebahattin Çürük

  2. Faculty of Engineering and Natural Sciences, Sabanci University, 81474, Tuzla, Istanbul, Turkey

    Selim Çetiner

  3. Faculty of Agriculture, Department of Horticulture, Çukurova University, 01330, Balcalı, Adana, Turkey

    Yeşim Yalçın-Mendi

  4. Institute of Plant Sciences, ARO Volcani Center, POB 6, Bet Dagan, 50250, Israel

    Mira Carmeli-Weissberg

  5. Department of Soil Chemistry, Plant Nutrition and Microbiology, ARO Volcani Center, POB 6, Bet Dagan, 50250, Israel

    Ellen Graber

  6. Department of Plant Pathology, ARO Volcani Center, POB 6, Bet Dagan, 50250, Israel

    Victor Gaba

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  1. Sebahattin Çürük
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  2. Selim Çetiner
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  3. Yeşim Yalçın-Mendi
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  4. Mira Carmeli-Weissberg
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  5. Ellen Graber
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  6. Victor Gaba
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Correspondence to Victor Gaba.

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Editor: David Duncan

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Çürük, S., Çetiner, S., Yalçın-Mendi, Y. et al. Food-grade sugar can promote differentiation in melon (Cucumis melo L.) tissue culture. In Vitro Cell.Dev.Biol.-Plant 48, 600–608 (2012). https://doi.org/10.1007/s11627-012-9453-0

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  • DOI: https://doi.org/10.1007/s11627-012-9453-0

Keywords

  • Sucrose quality
  • Food-grade sucrose
  • Bud regeneration
  • Melon
  • Cucumis melo