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Multiple inserts of gene of interest and selectable marker gene are co-integrated and stably transmitted as a single genetic locus in transgenic soybean plants

  • Plant Tissue Culture
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Abstract

Particle bombardment has been used for soybean transformation for more than 20 yr, but the integration and segregation of transgene inserts in the soybean genome have not been clearly documented. Over the past 5 yr, we processed several hundred transgenic events. In each experiment, the expression cassettes of the gene of interest (GOI) and hygromycin selectable marker gene (SMG) were co-bombarded into soybean at a 1:1 molecular ratio. More than 75% of hygromycin-resistant events also carried the GOI. Molecular analysis of transgenic plants revealed that most events carried multiple inserts of the GOI and the SMG. The GOI and the SMG were linked in selfed T1 and T2 progeny. Segregation analysis of progeny indicated that, in over 98% of the transgenic events, the multiple inserts of the GOI were integrated into the same genetic locus resulting in a 3:1 segregation ratio. Furthermore, the multiple inserts of the GOI are transmitted into succeeding generations, and no recombinants were found. These data indicate that in soybean plants, co-bombarded genes are preferentially integrated and stably segregated as a single genetic locus.

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Authors and Affiliations

  1. Targeted Growth, Inc., Ottawa, ON, K1A 0C6, Canada

    Rugang Li & Shuiqing Zhao

  2. Agriculture and Agri-Food Canada, Ottawa, ON, K1A 0C6, Canada

    Bonnie Bancroft, Suqin Zheng, Laureen Blahut-Beatty & Daina Simmonds

  3. Targeted Growth, Inc., Seattle, WA, 98013, USA

    Carolyn Hutcheon & Jay De Rocher

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  1. Rugang Li
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  2. Bonnie Bancroft
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  3. Carolyn Hutcheon
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  4. Shuiqing Zhao
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  5. Suqin Zheng
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  6. Laureen Blahut-Beatty
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  7. Jay De Rocher
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  8. Daina Simmonds
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Correspondence to Daina Simmonds.

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Editor: N. J. Taylor

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Li, R., Bancroft, B., Hutcheon, C. et al. Multiple inserts of gene of interest and selectable marker gene are co-integrated and stably transmitted as a single genetic locus in transgenic soybean plants. In Vitro Cell.Dev.Biol.-Plant 47, 274–281 (2011). https://doi.org/10.1007/s11627-011-9359-2

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  • DOI: https://doi.org/10.1007/s11627-011-9359-2

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