Abstract
Suaeda salsa calluses cultured in darkness for 28 d were transferred to Murashige and Skoog (MS) media containing various growth regulators under white light conditions for 10 d to investigate cell growth, betacyanin accumulation, and expression of dopa-4,5-dioxygenase (DODA). Callus growth was markedly promoted when 0.2 mg·L−1 2,4-D and 0.5 mg·L−1 6-BA were added to the MS medium. Surprisingly, of the auxins tested, IAA had no effect on betacyanin content, but 2,4-D strongly decreased betacyanin content. Betacyanin content was positively correlated with 6-BA concentrations in the range of 0.1–2.0 mg·L−1. DODA mRNA levels were consistent with the response of betacyanin content to exogenous growth regulators. These results suggest that betacyanin metabolism in S. salsa calluses is regulated under white light conditions by growth regulators through the regulation of genes such as DODA that are involved in betacyanin synthesis.
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Acknowledgments
This work was supported by grants from the National Natural Science Research Foundation of China (No. 30670177), the HI-Tech Research and Development (863) Program of China (No. 2007AA091701), and the Specialized Research Fund for the Doctoral Program of Higher Education of China (No. 20050445003). We thank an English-speaking professional with science background at Elixigen Corporation for a proofreading of the manuscript.
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Zhao, SZ., Sun, HZ., Gao, Y. et al. Growth regulator-induced betacyanin accumulation and dopa-4,5-dioxygenase (DODA) gene expression in euhalophyte Suaeda salsa calli. In Vitro Cell.Dev.Biol.-Plant 47, 391–398 (2011). https://doi.org/10.1007/s11627-011-9339-6
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DOI: https://doi.org/10.1007/s11627-011-9339-6