Abstract
Pineapple is one of the most important tropical fruits, but the availability of planting material is insufficient to agricultural demands. Therefore, several pineapple micropropagation protocols have been developed. However, acclimatization of in vitro plants continues to take a prolonged period. Biofertilizers have been found as safe alternatives to improve the agricultural performances of many crops. This study highlights some of the effects of the application of Azotobacter chroococcum (INIFAT5 strain) on in vitro pineapple plants during acclimatization. The bacteria were sprayed immediately after transplanting to the ex vitro environment; the plants were then sprayed every 4 wk. A control group of plants was established. Subsequently, after 5 mo, the evaluated variables included fresh and dry plant weight, plant height (cm), and root length (cm). The anatomy of middle-aged leaves and roots was also studied: transversal thickness and width of cuticle, epidermis, hypodermis, aquiferous parenchyma, and photosynthetic parenchyma. Thickness of root exoderm, external cortex, internal cortex, and stele were also evaluated. In general, the INIFAT5 strain improved the plant development. Results showed that the bacteria significantly provoked changes in the plant fresh weight, the thickness of the leaf abaxial and adaxial cuticles, and the root exoderm width. Contrastingly, A. chroococcum did not affect the thickness of the leaf photosynthetic parenchyma.
References
Bartholomew D. MD2 pineapple transforms the world’s pineapple fresh fruit export industry. Pineap. News 16: 2–5; 2009.
Botella J. R.; Fairbairn D. J. Present and future potential of pineapple biotechnology. Acta Hort. 622: 23–28; 2005.
Compant S.; Duffy B.; Nowak J.; Clement C.; Barka E. A. Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Appl. Environ. Microbiol. 71: 4951–4959; 2005.
Daquinta M.; Benega R. Brief review of tissue culture in pineapple. Pineap. News 3: 7–9; 1997.
Dibut A. B. Bio-fertilizers for sustainable agriculture. HUMIWORM, Mexico City, pp 11–34; 2005 (in Spanish).
Escalona M.; Lorenzo J. C.; González B.; Daquinta M.; González J. L.; Desjardins Y.; Borroto C. G. Pineapple (Ananas comosus (L.) Merr) micropropagation in temporary immersion systems. Plant Cell Rep. 18: 743–748; 1999.
FAOSTAT Available via: FAO STATISTIC DIVISION. http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID=567. Accessed 28 May 2009; 2008.
Johansen D. A. Plant microtechnique. McGraw-Hill Book, New York, p 528; 1940.
Khosravi H.; Samar S. M.; Fallahi E.; Davoodi H.; Shahabian M. Inoculation of ‘Golden Delicious’ apple trees on M9 rootstock with Azotobacter improves nutrient uptake and growth indices. J. Plant. Nutr. 32: 946–953; 2009.
Murashige T.; Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497; 1962.
Sashidhar B.; Podile A. R. Mineral phosphate solubilization by rhizosphere bacteria and scope for manipulation of the direct oxidation pathway involving glucose dehydrogenase. J. Appl. Microbiol. 109: 1–12; 2010.
Srivastava A. K.; Singh T.; Jana T. K.; Arora D. K. Induced resistance and control of charcoal rot in Cicer arietinum (chickpea) by Pseudomonas fluorescens. Can. J. Bot. 79: 787–795; 2001.
Wang L.; Uruu G.; Xiong L.; He X.; Nagai C.; Cheah T.; Hu S.; Nan G.; Sipes S.; Atkinson J.; Moore H.; Rohrbach G.; Paull R. Production of transgenic pineapple (Ananas comosus (L.) Merr.) plants via adventitious bud regeneration. In Vitro Cell Dev. Biol. Plant 45: 113–121; 2009.
Yanes E.; González J.; Rodríguez R. A technology of acclimatization of pineapple vitroplants. Pineap. News 7: 24; 2000.
Acknowledgments
This research was supported by the Cuban Ministry for Science, Technology and Environment and by the International Foundation for Science (Sweden) through grants to Rayza González. We are grateful to Yaima Pino, Fidel Durán, Diego Martínez, Julia Martínez, and Alitza Iglesias for their excellent technical assistance.
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González, R., Laudat, T., Arzola, M. et al. Effect of Azotobacter chroococcum on in vitro pineapple plants’ growth during acclimatization. In Vitro Cell.Dev.Biol.-Plant 47, 387–390 (2011). https://doi.org/10.1007/s11627-010-9334-3
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DOI: https://doi.org/10.1007/s11627-010-9334-3