Abstract
Cotton ovule cultures have obvious advantages over whole plants when experimental protocols call for inhibitors, radio-labeled precursors or controlled environmental conditions to be tested. The responses of ovule expansion growth and attached fiber elongation to external factors require accurate measurement techniques. This paper presents a new method for digital image analysis of the growth area of cotton ovules with fibers at high resolution. The method was characterized under constant conditions and during dynamic responses to different levels of ABA (abscisic acid) treatment. The growth area was treated as area occupied within the outline of the Petri dish image of the growing ovule with fibers. Growth area increase showed the same trends as fiber length increase and was significantly correlated with the fiber length increase under different levels of ABA treatment (r 2 = 0.97). This new analysis method provides a simple, noninvasive, and more accurate approach for growth analysis in the cotton ovule culture system. Using this method, the effects of ABA on expansion growth of ovule with fibers were characterized.
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Acknowledgments
The authors would like to thank Prof. Peter M Neumann (Technion IIT, Haifa, Israel) for manuscript revision. This research was supported by Hi-Tech Research and Development Program of Xinjiang, China (200611101), National Natural Science Foundation of China (30660088), and Hi-Tech Research and Development Program of China (2006AA10Z184).
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Editor: D. T. Tomes
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Fan, L., Lü, M., Ni, ZY. et al. Digital image analysis of expansion growth of cultured cotton ovules with fibers and their responses to ABA. In Vitro Cell.Dev.Biol.-Plant 47, 369–374 (2011). https://doi.org/10.1007/s11627-010-9329-0
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DOI: https://doi.org/10.1007/s11627-010-9329-0