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Anthocyanin production in a callus line of Panax sikkimensis Ban

  • Plant Tissue Culture
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Abstract

A root-derived callus line of Panax sikkimensis that stably accumulates anthocyanins was established by small cell aggregate selection method. The selected line showed a growth index of 221.36 and an anthocyanin content of 2.76 mg/g fw (7.076% dw) in 50–60 d of growth on a modified MS medium containing 4.5 µM 2,4-dichlorophenoxy acetic acid and 1.2 µM kinetin under 16-h light and 8-h dark photoperiodic conditions. Incubation under continuous light increased the growth index to 435.57 but led to a marginal dilution of anthocyanin content to 2.192 mg/g fw (6.928% dw). The purple-red pigment had absorption maximum at 528 nm. The selected callus line has shown sustained growth and productivity for more than 6 yr now. Interestingly, pigment accumulation in the selected line did not hinder the ginsenoside production in the callus tissue (0.9–1.2% fw).

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Acknowledgments

The authors wish to thank the Council of Scientific and Industrial Research (CSIR), New Delhi and Director CIMAP for the financial support and facilities for this work. AM also thanks the International Foundation for Science (IFS), Sweden for the partial funding to this investigation.

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Authors and Affiliations

  1. Plant Tissue Culture Division, Central Institute of Medicinal and Aromatic Plants, Council of Scientific & Industrial Research (CSIR), P.O. CIMAP, Lucknow, 226 015, India

    Archana Mathur, Ajay Kumar Mathur, Anita Gangwar, Sharawan Yadav & Priyanka Verma

  2. Plant Physiology and Biochemistry Division, Central Institute of Medicinal and Aromatic Plants, Council of Scientific & Industrial Research (CSIR), P.O. CIMAP, Lucknow, 226 015, India

    Rajender Singh Sangwan

Authors
  1. Archana Mathur
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  2. Ajay Kumar Mathur
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  3. Anita Gangwar
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  4. Sharawan Yadav
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  5. Priyanka Verma
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  6. Rajender Singh Sangwan
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Correspondence to Archana Mathur.

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Editor: D. T. Tomes

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Mathur, A., Mathur, A.K., Gangwar, A. et al. Anthocyanin production in a callus line of Panax sikkimensis Ban. In Vitro Cell.Dev.Biol.-Plant 46, 13–21 (2010). https://doi.org/10.1007/s11627-009-9253-3

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  • DOI: https://doi.org/10.1007/s11627-009-9253-3

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