Abstract
Passiflora alata in vitro organogenesis was studied based on explant type, culture medium composition, and incubation conditions. The results indicated that the morphogenic process occurred more efficiently when hypocotyl segment-derived explants were cultured in media supplemented with cytokinin and AgNO3 incubated under a 16-h photoperiod. The shoot bud elongation and plant development were obtained by transferring the material to MSM culture medium supplemented with GA3 and incubated in flasks with vented lids. Histological analyses of the process revealed that the difficulties in obtaining plants could be related to the development of protuberances and leaf primordia structures, which did not contain shoot apical meristem. Roots developed easily by transferring elongated shoots to 1/2 MSM culture medium. Plant acclimatization occurred successfully, and somaclonal variation was not visually detected. The efficiency of this organogenesis protocol will be evaluated for genetic transformation of this species to obtain transgenic plants expressing genes that can influence the resistance to Cowpea aphid borne mosaic virus.
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The authors acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), and Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for financial support and J. G. Brancalion for graphics assistance.
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Pinto, A.P.C., Monteiro-Hara, A.C.B.A., Stipp, L.C.L. et al. In vitro organogenesis of Passiflora alata. In Vitro Cell.Dev.Biol.-Plant 46, 28–33 (2010). https://doi.org/10.1007/s11627-009-9251-5
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DOI: https://doi.org/10.1007/s11627-009-9251-5