Skip to main content
SpringerLink
Log in

In vitro adventitious shoot regeneration of the medicinal plant meadowsweet (Filipendula ulmaria (L.) Maxim)

  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

Filipendula ulmaria (L.) Maxim (meadowsweet) is a medicinal plant that is claimed to have several biological activities, including anti-tumor, anti-carcinogenic, anti-oxidant, anti-coagulant, anti-ulcerogenic, anti-microbial, anti-arthritic, and immunomodulatory properties. This report describes, for the first time, an efficient plant regeneration system for F. ulmaria via adventitious shoot development from leaf, petiole, and root explants cultured on Murashige and Skoog’s minimal organics medium containing different concentrations of thidiazuron (TDZ), benzyladenine, and kinetin either alone or in combination with different auxins. Relatively extensive/prolific shoot regeneration was observed in all three explant types with TDZ in combination with indole-3-acetic acid (IAA). Gibberellic acid (GA3), TDZ, and IAA combinations were also tested. The best shoot proliferation was observed among root explants cultured on media supplemented with 0.45 μM TDZ + 2.85 μM IAA + 1.44 μM GA3. Regenerated shoots were transferred to rooting media containing different concentrations of either IAA, indole-3-butyric acid (IBA), naphthalene acetic acid, or 2,4-dichlorophenoxyacetic acid. Most shoots developed roots on medium with 2.46 μM IBA. Rooted explants were transferred to vermiculite in Magenta containers for a 2-wk acclimatization period and then finally to plastic pots containing potting soil. The plantlets in soil were kept in growth chambers for 2 wk before transferring to greenhouse conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.

Similar content being viewed by others

References

  • Ahn Y. J.; Vang L.; McKeon T. A.; Chen G. Q. High-frequency plant regeneration through adventitious shoot formation in castor (Ricinus communis L.). In Vitro Cell. Dev. Biol. Plant 43: 9–15; 2007. . doi:10.1007/s11627-006-9009-2.

    Article  CAS  Google Scholar 

  • Baker H. G.; Baker I. The cytotaxonomy of Filipendula (Rosaceae) and its implications. Am. J. Bot 54: 1027–1034; 1967. doi:10.2307/2440727.

    Article  Google Scholar 

  • Barnaulov O. D.; Denisenko P. P. Antiulcerogenic action of the decoction from flowers of Filipendula-ulmaria (L.) Maxim. Farmakol. Toksikol 43: 700–705; 1980.

    PubMed  CAS  Google Scholar 

  • Barnaulov O. D.; Manicheva O. A.; Shelyuto V. L.; Konopleva M. M.; Glyzin V. I. Effect of flavonoids on the development of experimental gastric dystrophies in mice. Khim. Farm. Zh 18: 935–941; 1984.

    CAS  Google Scholar 

  • Bespalov V. G.; Limarenko A. Y.; Voitenkov B. O.; Petrov A. S.; Troyan D. N.; Aleksandrov V. A.; Peresunko A. P. Anticarcinogenic, antitumor, and modulating properties of dropwort (Filipendula ulmaria) flower decoction. Khim. Farm. Zh 26: 59–61; 1992.

    CAS  Google Scholar 

  • Chaturvedi H. C.; Jain M.; Kidwai N. R. Cloning of medicinal plants through tissue culture-A review. Indian J. Exp. Biol 45: 937–948; 2007.

    PubMed  CAS  Google Scholar 

  • Chevallier A. The encyclopedia of medicinal plants. Dorling Kindersley, London, p 96; 1996.

    Google Scholar 

  • Csedo K.; Monea M.; Sabau M.; Esianu S. The antibiotic activity of Filipendula ulmaria. Planta Med 59: A675; 1993. doi:10.1055/s-2006-959953.

    Article  Google Scholar 

  • Davis P. H. Flora of Turkey and the East Aegean Islands, vol. 4. Edinburgh Univ. Press, Edinburgh, p 29; 1972.

    Google Scholar 

  • De Gyves E. M.; Sparks C. A.; Fieldsend A. F.; Lazzeri P. A.; Jones H. D. High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron. Ann. Appl. Biol 138: 329–332; 2001. doi:10.1111/j.1744-7348.2001.tb00117.x.

    Article  Google Scholar 

  • Faisal M.; Siddique I.; Anis M. In vitro rapid regeneration of plantlets from nodal explants of Mucuna pruriens—a valuable medicinal plant. Ann. Appl. Biol 148: 1–6; 2006. doi:10.1111/j.1744-7348.2005.00034.x.

    Article  CAS  Google Scholar 

  • Geetha N.; Venkatachalam P.; Prakash V.; Sita G. L. High frequency induction of multiple shoots and plant regeneration from seedling explants of pigeonpea (Cajanus cajan L.). Curr. Sci 75: 1036–1041; 1998.

    CAS  Google Scholar 

  • Grieve M. A modern herbal, vol. 2. Dover, New York, p 524; 1982.

    Google Scholar 

  • Halkes S. B. A.; Beukelman C. J.; Kroes B. H.; van den Berg A. J. J.; Labadie R. P.; Van Dijk H. In vitro immunomodulatory activity of Filipendula ulmaria. Phytother. Res 11: 518–520; 1997. doi:10.1002/(SICI)1099-1573(199711)11:7<518::AID-PTR136>3.0.CO;2-9.

    Article  Google Scholar 

  • Huetteman C. A.; Preece J. E. Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ. Cult 33: 105–119; 1993.

    Article  CAS  Google Scholar 

  • Jasiuleviciute L.; Keturkiene A.; Leonaviciene L. Relation between zinc and magnesium content in liver of rats with adjuvant arthritis and treatment with the tincture of Filipendula ulmaria (L.) Maxim. Acta Med. Litu. 8: 4; 2001.

    Google Scholar 

  • Jones M. P. A.; Yi Z. J.; Murch S. J.; Saxena P. K. Thidiazuron-induced regeneration of Echinacea purpurea L.: Micropropagation in solid and liquid culture systems. Plant Cell Rep 26: 13–19; 2007. doi:10.1007/s00299-006-0209-3.

    Article  PubMed  CAS  Google Scholar 

  • Krasnov E. A.; Raldugin V. A.; Shilova I. V.; Avdeeva E. Phenolic compounds from Filipendula ulmaria. Chem. Nat. Compd 42: 148–151; 2006. doi:10.1007/s10600-006-0064-z.

    Article  CAS  Google Scholar 

  • Kudrjashov B. A.; Lyapina L. A.; Aziyeva L. D. Heparin-like anticoagulant content in Filipendula - ulmaria flowers. Farmakol. Toksikol 53: 39–41; 1990.

    Google Scholar 

  • Lata H.; Bedir E.; Hosick A.; Ganzera M.; Khan I.; Moraes R. M. In vitro plant regeneration from leaf-derived callus of Cimicifuga racemosa. Planta Med 68: 912–915; 2002. doi:10.1055/s-2002-34933.

    Article  PubMed  CAS  Google Scholar 

  • Lindeman A.; Jounela-Eriksson P.; Lounasmaa M. The aroma composition of the flower of meadowsweet (Filipendula ulmaria (L.) Maxim.). Lebensm-Wiss. Technol 15: 286–289; 1982.

    CAS  Google Scholar 

  • Lombardi S. P.; Passos I. R. S.; Nogueira M. C. S.; Glória B. A. In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata Mast. Braz. Arch. Biol. Techn 50: 239–247; 2007.

    Google Scholar 

  • Lu C. Y. The use of TDZ in tissue culture. In vitro Cell. Dev. Biol. Plant 29: 92–96; 1993. doi:10.1007/BF02632259.

    Article  Google Scholar 

  • Lyapina L. A.; Kovalchuk G. A. A comparative-study of the effects of Filipendula-ulmaria flower and seed extracts on hemostasis. Izv. Akad. Nauk. Biol 4: 625–628; 1993.

    Google Scholar 

  • Murashige T.; Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum 15: 473–497; 1962. doi:10.1111/j.1399-3054.1962.tb08052.x.

    Article  CAS  Google Scholar 

  • Papp I.; Apati P.; Andrasek V.; Blazovics A.; Balazs A.; Kursinszki L.; Kite G. C.; Houghton P. J.; Kery A. LC-MS analysis of antioxidant plant phenoloids. Chromatographia 60: S93–S100; 2004. doi:10.1365/s10337-004-0348-z.

    Article  CAS  Google Scholar 

  • Peresunko A. P.; Bespalov V. G.; Limarenko A. I.; Aleksandrov V. A. Clinico-experimental study of using plant preparations from the flowers of Filipendula ulmaria (L.) Maxim for the treatment of precancerous changes and prevention of uterine cervical cancer. Vopr. Onkol 39: 291–295; 1993.

    CAS  Google Scholar 

  • Rauha J. P.; Remes S.; Heinonen M.; Hopia A.; Kähkönen M.; Kujala T.; Pihlaja K.; Vuorela H.; Vuorela P. Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int. J. Food Microbiol 56: 3–12; 2000. doi:10.1016/S0168-1605(00)00218-X.

    Article  PubMed  CAS  Google Scholar 

  • Rout G. R.; Samantaray S.; Das P. In vitro manipulation and propagation of medicinal plants. Biotechnol. Adv 18: 91–120; 2000. doi:10.1016/S0734-9750(99)00026-9.

    Article  PubMed  CAS  Google Scholar 

  • Schwarz, O. J.; Beaty, R. M. Propagation from nonmeristematic tissues-organogenesis. In: Trigiano, R. N.; Gray, D. J. (eds). Plant tissue culture concepts and laboratory exercises. CRC, Boca Raton, pp 95–103; 1996.

    Google Scholar 

  • Shahzad A.; Faisal M.; Anis M. Micropropagation through excised root culture of Clitoria ternatea and comparison between in vitro-regenerated plants and seedlings. Ann. Appl. Biol 150: 341–349; 2007. doi:10.1111/j.1744-7348.2007.00132.x.

    Article  CAS  Google Scholar 

  • Siddique I.; Anis M. In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia Vahl.: a medicinal plant. Acta Physiol. Plant 29: 233–238; 2007. doi:10.1007/s11738-007-0029-2.

    Article  CAS  Google Scholar 

  • Singh N. D.; Sahoo L.; Sarin N. B.; Jaiwal P. K. The effect of TDZ on organogenesis and somatic embryogenesis in pigeonpea (Cajanus cajan L. Millsp). Plant Sci 164: 341–347; 2003. doi:10.1016/S0168-9452(02)00418-1.

    Article  CAS  Google Scholar 

  • Sroka Z.; Cisowski W.; Seredynska M.; Luczkiewicz M. Phenolic extracts from meadowsweet and hawthorn flowers have antioxidative properties. Z. Naturforsch. C 56: 739–744; 2001.

    PubMed  CAS  Google Scholar 

  • Sutter, E. G. General laboratory requirements, media and sterilization methods. In: Trigiano, R. N.; Gray, D. J. (eds). Plant tissue culture concepts and laboratory exercises. CRC, Boca Raton; 1996: pp 18.

    Google Scholar 

  • Valle M. G.; Nano G. M.; Tira S. The essential oil of Filipendula ulmaria. Planta Med 2: 181–182; 1988. doi:10.1055/s-2006-962390.

    Article  Google Scholar 

  • Vasiliauskas A.; Keturkienei A.; Leonavieiene L.; Vaitkiene D. Influence of herb Filipendula ulmaria (L.) Maxim tincture on pro-/ antioxidant status in gastric tissue with indomethacin-induced gastric ulser in rats. Acta Med. Litu 11: 31–36; 2004.

    Google Scholar 

  • Wang J.; Bao M. Z. Plant regeneration of pansy (Viola wittrockiana) ‘Caidie’ via petiole-derived callus. Sci. Hortic 111: 266–270; 2007. doi:10.1016/j.scienta.2006.10.011.

    Article  CAS  Google Scholar 

  • Weidema I. R.; Magnussen L. S.; Philipp M. Gene flow and mode of pollination in a dry-grassland species, Filipendula vulgaris (Rosaceae). Heredity 84: 311–320; 2000. doi:10.1046/j.1365-2540.2000.00669.x.

    Article  PubMed  CAS  Google Scholar 

  • Xiao X. G.; Branchard M. In vitro high frequency plant regeneration from hypocotyl and root segments of spinach by organogenesis. Plant Cell Tissue. Organ Cult 42: 239–244; 1995.

    Article  CAS  Google Scholar 

  • Yucesan B.; Turker A. U.; Gurel E. TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.). Plant Cell Tissue Organ Cult 91: 243–250; 2007.

    Article  CAS  Google Scholar 

  • Zeylstra H. Filipendula ulmaria. Br. J. Phytother 5: 8–12; 1998.

    Google Scholar 

  • Zobayed S. M. A.; Saxena P. K. In vitro-grown roots: a superior explant for prolific shoot regeneration of St. John’s wort (Hypericum perforatum L. cv ‘New Stem’) in a temporary immersion bioreactor. Plant Sci 165: 463–470; 2003. doi:10.1016/S0168-9452(03)00064-5.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to The Scientific and Technological Research Council of Turkey (TUBITAK) for financial support [TBAG-2278(103T024) and TBAG-HD/27(105T040)]. We are grateful to Dr. Hakan Turker for his technical support.

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Arts and Sciences, Abant Izzet Baysal University, Bolu, Turkey

    A. B. Yıldırım & A. U. Turker

Authors
  1. A. B. Yıldırım
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. U. Turker
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. U. Turker.

Additional information

Editor: N.J. Taylor

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yıldırım, A.B., Turker, A.U. In vitro adventitious shoot regeneration of the medicinal plant meadowsweet (Filipendula ulmaria (L.) Maxim). In Vitro Cell.Dev.Biol.-Plant 45, 135–144 (2009). https://doi.org/10.1007/s11627-009-9194-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-009-9194-x

Keywords

Search

Navigation