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Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants

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Abstract

Arbutus unedo L. is a species of strawberry tree, widely represented in the Mediterranean climates of southern Europe. Fruits are used to make jellies and a spirit called “medronheira.” Shoot apices and nodal segments from epicormic and coppiced shoots of adult plants were used for plant propagation. Shoot apices from epicormic shoots, which were developed in a growth chamber, showed higher rates of in vitro establishment. The results also indicated that shoot apices are more effective for plant establishment than nodal segments, with rates of establishment significantly higher after 12 wk of culture. Of the three basal media used in combination with 9.0 µM benzyladenine and 0.087 M sucrose, the FS medium with the micronutrients of the Murashige and Skoog medium gave the highest rates of multiplication, especially when the parameter analyzed was the number of clusters formed. When shoot apices from selected adult plants (AL01–AL06) were tested, the multiplication rate was not significantly different among the plants. However, in the conditions tested, shoots from the clones AL1, AL2, and AL3 showed better development, whereas shoots from AL4, AL5, and AL6 showed an impaired development and could not be rooted. Rooting was achieved in all the conditions tested, even in the absence of auxin. The inclusion of an auxin significantly increased root formation, whereas the addition of charcoal did not improve root formation. Rooted plantlets were acclimatized, and some of them are now in the field for further study.

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References

  • Anderson, W. C. A revised tissue culture medium for shoot multiplication of Rhododendron. J. Amer. Soc. Hort. Sci. 109: 343–347; 1984.

    Google Scholar 

  • Arezki O.; Boxus P.; Kevers C.; Gaspar T. Hormonal control of proliferation in meristematic agglomerates of Eucalyptus camaldulensis Dehn. In Vitro Cell Dev. Biol. Plant 36: 398–401; 2000. doi:10.1007/s11627-000-0071-x.

    Article  CAS  Google Scholar 

  • Banko, T. J.; Stefani, M. A. In vitro propagation of Oxydendrum arboreum from mature trees. HortScience 24: 683–685; 1989.

    Google Scholar 

  • Bennett, I. J.; McComb, J. A.; Tonkin, C. M.; Mcdavid, D. A. J. Alternating cytokinins in multiplication media stimulates in vitro shoot growth and rooting of Eucalyptus globulus Labill. Ann. Bot. 74: 53–58; 1994. doi:10.1006/anbo.1994.1093.

    Article  CAS  Google Scholar 

  • Canhoto, J. M.; Lopes, M. L.; Cruz, G. S. Somatic embryogenesis in myrtaceous plants. In: Jain S. M.; Gupta P. K.; Newton R. J. (eds) Somatic embryogenesis in woody plants, vol 4. Kluwer, Dordrecht, pp 293–340; 1999.

    Google Scholar 

  • Canhoto, J. M.; Lopes, M. L.; Sequeira, J.; Gomes, F. Somatic embryogenesis and organogenesis induction from adult trees of A. unedo and A. canariensis. IUFRO Tree Biotechnology, Azores, p 12; 2007.

    Google Scholar 

  • Cao, X.; Fordahm, I.; Douglas, L.; Hammerschlag, F. A. Sucrose levels influences micropropagation and gene delivery into leaves from in vitro propagated highbush blueberry shoots. Plant Cell Tiss. Org. Cul. 75: 255–259; 2003. doi:10.1023/A:1025856404937.

    Article  CAS  Google Scholar 

  • Cardoso, A. V. R. Historial recente da propagação vegetativa do medronheiro no Algarve Contribuição para a criação de um futuro parque de pés-mãe. Graduation Thesis, ISA-UTL, Lisboa; 2004.

  • Dal Vesco, L. L.; Guerra, M. P. The effectiveness of nitrogen sources in Feijoa somatic embryogenesis. Plant Cell Tiss. Org. Cult. 64: 19–25; 2001. doi:10.1023/A:1010635926146.

    Article  Google Scholar 

  • De Fossard, R. A.; Nitsch, C.; Cresswell, R. J.; Lee, H. C. M. Tissue and organ culture of Eucalyptus. N. Z. J. For. Sci. 4: 267–278; 1974.

    Google Scholar 

  • Duncan, D. B. Multiple range and multiple F tests. Biometry 11: 1–42; 1955. doi:10.2307/3001478.

    Article  Google Scholar 

  • Ferreira, C.; Dias, J. D.; Canhoto, J. M. In vitro propagation of Leucadendron laureolum x L. salignum cv. Safari Sunset and cytological analysis of the regenerated plantlets. Acta Hortic. 602: 29–38; 2003.

    Google Scholar 

  • Gajdošová, A.; Ostrolucká, M. G.; Libiaková, G.; Ondrušková, E. Protocol for micropropagation of Vaccinium vitis-idaea L. In: Jain S. M.; Häggman (eds) Protocols for micropropagation of woody trees and fruits. Springer, Berlin, pp 457–464; 2007.

    Google Scholar 

  • Gautheret, R. J. La culture des tissues végétaux, techniques et réalisations. Masson, Paris1959.

    Google Scholar 

  • Gomes, F.; Canhoto, J. M. Micropropagation of Eucalyptus nitens Maiden (Shining gum). In Vitro Cell Dev. Biol. Plant 39: 316–321; 2003. doi:10.1079/IVP2002376.

    Article  CAS  Google Scholar 

  • Gonzalez, M. V.; Lopez, M.; Valdes, A. E.; Ordas, R. J. Micropropagation of three berry fruit species using nodal segments from field-grown plants. Ann. Appl. Biol. 137: 73–78; 2000. doi:10.1111/j.1744-7348.2000.tb00059.x.

    Article  CAS  Google Scholar 

  • Hackett, W. P. Juvenility, maturation and rejuvenation in woody plants. Hortic. Rev. 7: 109–155; 1985.

    Google Scholar 

  • Hansen, E.; Olsen, J. E.; Junttila, O. Gibberellins and subapical cell divisions in relation to bud set and bud break in Salix pentrana. J. Plant Growth Reg. 18: 167–170; 1999. doi:10.1007/PL00007065.

    Article  CAS  Google Scholar 

  • Hartman, H. T.; Kester, D.; Davies, F. T. Jr; Geneve, R. L. Plant propagation: principles and practices, 6th edn. Prentice Hall Int, Upper Saddle; 1997.

  • Heywood, V. H. Flowering plants of the world. B.T. Batsford, Londres; 1993.

    Google Scholar 

  • Ibañez, A.; Valero, M.; Morte, A. Establishment and in vitro clonal propagation of the Spanish autochthonous table grapevine cultivar Napoleon: an improved system where proliferating cultures alternate with rooting ones. Ann. Biol. 27: 211–220; 2005.

    Google Scholar 

  • Isutsa, D. K.; Pritts, M. P.; Mudge, K. W. Rapid propagation of blueberry plants using ex-vitro rooting and controlled acclimatization of micropropagules. HortScience 29: 1124–1126; 1994.

    Google Scholar 

  • Jain, S. M.; Häggman, H. Protocols for micropropagation of woody trees and fruits. Springer, Berlin; 2007.

    Book  Google Scholar 

  • Kohlenbach, H. W.; Wernicke, W. Investigations on the inhibitory effect of agar and the function of active carbon in anther culture. Z. Pflanzenphysiol. 84: 463–472; 1978.

    Google Scholar 

  • Lloyd, G. B.; McCown, B. H. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proc. Int. Plant Prop. Soc. 30: 421–427; 1980.

    Google Scholar 

  • Mackay, W. A. Micropropagation of Texas madrone, Arbutus xalapensis HBK. HortScience 31: 1028–1029; 1996.

    Google Scholar 

  • McComb, J. A.; Bennett, I. J. Eucalypts (Eucalyptus spp.). In: Bajaj Y. P. S. (ed) Biotechnology in agriculture and forestry vol 1. Trees I. Springer, Berlin, pp 340–362; 1986.

    Google Scholar 

  • Mendes, M. L. A. Multiplicação vegetativa in vitro de medronheiro. Master Thesis, ISA-UTL, Lisboa; 1997.

  • Mereti, M.; Grigoriadou, K.; Nanos, G. D. Micropropagation of the strawberry tree, Arbutus unedo L. Sci. Hortic. 93: 143–148; 2002. doi:10.1016/S0304-4238(01)00330-2.

    Article  CAS  Google Scholar 

  • Metaxas, D. J.; Syros, T. D.; Yupsanis, T.; Economou, A. E. Peroxidases during adventitious rooting in cuttings of Arbutus unedo and Taxus baccata as affected by plant genotype and growth regulator treatment. Plant Growth Regul. 44: 257–266; 2004. doi:10.1007/s10725-004-5931-7.

    Article  CAS  Google Scholar 

  • Murashige, T. Plant propagation through tissue culture. Ann. Rev. Plant Physiol. 25: 135–166; 1974. doi:10.1146/annurev.pp.25.060174.001031.

    Article  CAS  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco cultures. Physiol. Plant 15: 473–497; 1962. doi:10.1111/j.1399-3054.1962.tb08052.x.

    Article  CAS  Google Scholar 

  • Mylona, P.; Dolan, L. The root meristem. In: McManus M. T.; Weit B. E. (eds) Meristematic tissues in plant growth and development. Sheffield Academic, Sheffield, pp 279–292; 2002.

    Google Scholar 

  • Neppi, M. Alberi ed arbusti della flora mellifera della regione mediterrânea. In: Piotto, B.; Noi, A. (eds) Propagazione per seme di alberi e arbusti della flora mediterranea, Dipartimento Prevenzione e Risanamento Ambientali, Manuale ANPA (Agenz. Naz. per la Protezi. dell’Ambiente), pp 44–49; 2001.

  • Niedz, R. P. Growth of embryogenic sweet orange callus on media varying in the ratio of nitrate to ammonium nitrogen. Plant Cell Tiss. Org. Cul. 39: 1–5; 1994. doi:10.1007/BF00037584.

    Article  CAS  Google Scholar 

  • Ostrolucká, M. G.; Gajdošová, A.; Libiaková, G.; Hrubíková, K.; Bezo, M. Protocol for micropropagation of selected Vaccinium. In: Jain S. M.; Häggman (eds) Protocols for micropropagation of woody trees and fruits. Springer, Berlin, pp 445–455; 2007.

    Chapter  Google Scholar 

  • Pabuccuoglu, A.; Kivcak, B.; Bas, M.; Mert, T. Antioxidant activity of Arbutus unedo leaves. Fitoterapia 74: 597–599; 2003. doi:10.1016/S0367-326X(03)00110-2.

    Article  PubMed  CAS  Google Scholar 

  • Panteleitchouk, A. V. Micropropagação de alfarrobeira (Ceratonia siliqua L.) variedade Aida. Estudos químicos e de microscopia. Master Thesis, University of Coimbra, Portugal; 2002.

  • Piotto, B., Piccini, C., Arcadu, P. La ripresa della vegetazione dopo gli incendi nella regione mediterrânea. In: Piotto, B.; Noi, A. (eds) Propagazione per seme di alberi e arbusti della flora mediterranea, Dipartimento Prevenzione e Risanamento Ambientali, Manuale ANPA (Agenz. Naz. per la Protezi. dell’Ambiente), pp 32–38; 2001.

  • Rathore, J. S.; Rathore, V.; Shekhawat, N. S.; Singh, R. P.; Liler, G.; Mahendra, P.; Dagla, H. R. Micropropagation of woody plants. In: Srivastava P. S.; Narula A.; Srivastava S. (eds) Plant biotechnology and molecular markers. Anamaya, New Dehli, pp 195–205; 2004.

    Google Scholar 

  • Salisbury, F. B.; Ross, C. W. Plant physiology. 4th ed. Wadsworth, Belmont; 1992.

    Google Scholar 

  • Torres, J. A.; Valle, F.; Pinto, C.; Garcia-Fuentes, A.; Salazar, C.; Cano, E. Arbutus unedo communities in southern Iberian Peninsula Mountains. Plant Ecol. 160: 207–223; 2002. doi:10.1023/A:1015864821706.

    Article  Google Scholar 

  • Young, M. J.; Cameraon, J. S. Influence of growth regulators and nitrogen form on micropropagation of rabbiteye blueberries. Fruit Var. J. 39: 16–18; 1985.

    Google Scholar 

  • Zar, J. H. Biostatistical analysis. 3rd ed. Prentice-Hall, New Jersey; 1996.

    Google Scholar 

  • Ziv, M. In vitro hardening and acclimatization of tissue culture plants. In: Withers L. A.; Alderson P. G. (eds) Plant tissue culture and its agricultural application. Butterworth, London, pp 187–196; 1986.

    Google Scholar 

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Authors and Affiliations

  1. CERNAS, Departamento Florestal, Escola Superior Agrária de Coimbra, Bencanta, 3040-316, Coimbra, Portugal

    Filomena Gomes

  2. Plant Biotechnology Laboratory, Centre for Pharmaceutical Research, Department of Botany, University of Coimbra, 3001-455, Coimbra, Portugal

    Jorge M. Canhoto

  3. Departamento de Botânica, FCTUC, Calçada Martim de Freitas, 3001-455, Coimbra, Portugal

    Jorge M. Canhoto

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  1. Filomena Gomes
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  2. Jorge M. Canhoto
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Correspondence to Jorge M. Canhoto.

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Editor: Gregory C. Phillips

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Gomes, F., Canhoto, J.M. Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants. In Vitro Cell.Dev.Biol.-Plant 45, 72–82 (2009). https://doi.org/10.1007/s11627-008-9164-8

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  • DOI: https://doi.org/10.1007/s11627-008-9164-8

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