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The effect of auxin type and cytokinin concentration on callus induction and plant regeneration frequency from immature inflorescence segments of seashore paspalum (Paspalum vaginatum Swartz)

  • Developmental Biology/Morphogenesis
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Abstract

Seashore paspalum (Paspalum vaginatum Swartz) is a salt tolerant, fine textured turfgrass used on golf courses in coastal, tropical, and subtropical regions. A callus induction and plant regeneration protocol for this commercially important turfgrass species has been developed. Induction of highly regenerable callus with approximately 400 shoots per cultured immature inflorescence (1 cm in length) was achieved by culturing 0.2 cm segments on media with 3 mg l−1 3,6-dichloro-2-methoxybenzoic acid (dicamba) and 0.1 or 1.0 mg l−1 benzylaminopurine (BA). A multifactorial experiment demonstrated the combination of 3 mg l−1 dicamba and 1.0 mg l−1 BA for induction of callus resulted in 12 times higher plant regeneration frequency compared to 3 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) alone or ten times higher plant regeneration frequency than the combination of 3 mg l−1 2,4-D and 1.0 mg l−1 BA. These results are expected to support the development of a genetic transformation protocol for seashore paspalum.

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Acknowledgements

We thank Erika Erickson and Sima Patel for excellent technical assistance. We would also like to thank Turfgrass America, Houston, Texas, USA for provision of sod from Seashore paspalum cultivar Sea Isle 1. We are grateful for financial support from USDA-CSREES T-STAR.

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Authors and Affiliations

  1. Agronomy Department, Plant Molecular and Cellular Biology Program, Genetics Institute, University of Florida—IFAS, 2191 McCarty Hall, Gainesville, FL, 32611, USA

    Isaac Neibaur, Maria Gallo & Fredy Altpeter

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  1. Isaac Neibaur
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  2. Maria Gallo
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  3. Fredy Altpeter
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Correspondence to Fredy Altpeter.

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Gregory C. Phillips

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Neibaur, I., Gallo, M. & Altpeter, F. The effect of auxin type and cytokinin concentration on callus induction and plant regeneration frequency from immature inflorescence segments of seashore paspalum (Paspalum vaginatum Swartz). In Vitro Cell.Dev.Biol.-Plant 44, 480–486 (2008). https://doi.org/10.1007/s11627-008-9143-0

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