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Effect of cytokinins on in vitro development of autotrophism and acclimatization of Annona glabra L.

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Abstract

The role of cytokinins in the differentiation of the photosynthetic apparatus in micropropagated plants and their effect on the plant’s ability to transition from a heterotrophic to an autotrophic condition during acclimatization was investigated. Annona glabra L. shoots were cultured on woody plant medium supplemented with sucrose and different cytokinins to evaluate leaf tissue for chloroplast development, chloroplast numbers, photosynthetic pigmentation, total photosynthetic potential, and soluble sugar content. Plants were transferred to the rooting medium in the presence or absence of sucrose and then acclimatized. Kinetin and benzyladenine (BAP) stimulated chloroplast differentiation. Inclusion of zeatin in the medium induced the formation of greater numbers of chloroplasts in the leaves, while plants cultivated in the presence of only kinetin and BAP demonstrated greater chlorophyll a and carotenoid content. The use of kinetin and BAP during in vitro culture promoted accumulation of dry matter during the acclimatization phase, especially in plants rooted under autotrophic conditions (without sucrose). Kinetin and BAP promoted development of more leaf area and greater plant survival rates in plant acclimatization on both autotrophic and heterotrophic media. The inhibitory effects of thidiazuron on the differentiation of chloroplasts, accumulation of chlorophyll a, and photosynthetic potential were examined.

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Acknowledgments

The authors would like to thank the Research Foundation of the State of Minas Gerais (FAPEMIG) for their financial support.

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Authors and Affiliations

  1. Department of Biological Sciences, State University of Feira de Santana—UEFS, Av. Universitária, s/n, Feira de Santana, BA, 44031-060, Brazil

    Lenaldo Muniz de Oliveira, José Raniere Ferreira de Santana & Flávia Dionísio Pereira

  2. Department of Biology, Federal University of Lavras—UFLA, Cx. P. 37, Lavras, MG, 37200–000, Brazil

    Renato Paiva & Rairys Cravo Nogueira

  3. Department of Phytopathology, Federal University of Lavras, Cx. P. 37, Lavras, MG, 37200–000, Brazil

    Eduardo Alves

Authors
  1. Lenaldo Muniz de Oliveira
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  2. Renato Paiva
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  3. José Raniere Ferreira de Santana
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  4. Eduardo Alves
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  5. Rairys Cravo Nogueira
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  6. Flávia Dionísio Pereira
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Correspondence to Lenaldo Muniz de Oliveira.

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Editor: S. Mohan Jain

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de Oliveira, L.M., Paiva, R., de Santana, J.R.F. et al. Effect of cytokinins on in vitro development of autotrophism and acclimatization of Annona glabra L.. In Vitro Cell.Dev.Biol.-Plant 44, 128–135 (2008). https://doi.org/10.1007/s11627-008-9119-0

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  • DOI: https://doi.org/10.1007/s11627-008-9119-0

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