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Effects of genotype, explant source, and plant growth regulators on indirect shoot organogenesis in Dieffenbachia cultivars

  • Developmental Biology/Morphogenesis
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Abstract

The capacity for indirect shoot organogenesis of leaf and root explants of four Dieffenbachia cultivars were examined on a modified Murashige and Skoog (MS; Physiol Plant 15:473–495, 1962) medium supplemented with different plant growth regulators in 112 combinations. Callus formation was only observed from leaf explants on MS supplemented with 1–10 μM thidiazuron (TDZ) and 0.5–1.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D) regardless of cultivars. The combination of 5 μM TDZ and 1 μM 2,4-D resulted in the greatest callus formation frequency among the four cultivars tested. Significant differences in callus and shoot formation from leaf explants were also observed among cultivars. Cultivars Camouflage, Camille, Octopus, and Star Bright produced green nodular, brown nodular, yellow friable, and green compact calli with corresponding maximum callus formation frequencies of 96%, 62%, 54%, and 52%, respectively. A maximum of 6.7 shoots/callus was observed in cv. Camouflage, followed by cvs. Camille and Star Bright at 3.7 and 3.5, respectively. Calli of cv. Octopus displayed no capacity for shoot organogenesis. Regardless of cultivar, callus formation was not observed on root explants. Regenerated shoots were successfully acclimatized in a shaded greenhouse condition with 100% survival.

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Authors and Affiliations

  1. Department of Environmental Horticulture, Institute of Food and Agricultural Science, University of Florida, Gainesville, FL, 32611-0675, USA

    Xiuli Shen & Michael E. Kane

  2. Department of Environmental Horticulture and Mid-Florida Research & Education Center, Institute of Food and Agricultural Science, University of Florida, 2725 Binion Road, Apopka, FL, 32703-8504, USA

    Jianjun Chen

Authors
  1. Xiuli Shen
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  2. Michael E. Kane
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  3. Jianjun Chen
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Correspondence to Xiuli Shen.

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Editor: Gregory C. Philips

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Shen, X., Kane, M.E. & Chen, J. Effects of genotype, explant source, and plant growth regulators on indirect shoot organogenesis in Dieffenbachia cultivars. In Vitro Cell.Dev.Biol.-Plant 44, 282–288 (2008). https://doi.org/10.1007/s11627-008-9112-7

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  • DOI: https://doi.org/10.1007/s11627-008-9112-7

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