Abstract
High-frequency plant regeneration of C. roseus cv. ‘little bright eye’ via somatic embryogenesis and organogenesis from five out of six explants was standardized. Two factors were found to be important for regeneration: (1) the type of explants, and (2) the combination and concentrations of plant growth regulators. The highest regeneration percentage through somatic embryogenesis was obtained from mature zygotic embryo in MS medium supplemented with 7.5 μM of thidiazuron (TDZ). The mature embryo also regenerated efficiently via organogenesis in MS medium supplemented with either 2.5 μM TDZ or 5.3 μM α-naphthalene acetic acid (NAA) and 2.2 μM 6-benzylaminopurine (BA). Hypocotyl and cotyledon did not induce somatic embryogenesis and organogenesis in TDZ-containing medium but gave a maximum percentage of shoots in MS medium supplemented with 5.3 μM NAA and 2.2 μM BA. Stem nodes and meristem tips showed better regeneration via organogenesis in the medium supplemented with NAA and BA and in lower concentrations of TDZ.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bhadra, R.; Vani, S.; Shanks, J. V. Production of indole alkaloids by selected hairy root lines of Catharanthus roseus. Biotechnol. Bioeng. 41:581–592; 1993.
Choi, P. S.; Lee, S. Y.; Chung, H. J.; In, D. S.; Choi, D. W.; Liu, J. R. Assessment of competence for adventitious shoot formation in hypocotyl explant cultures of 22 cultivars of Catharanthus roseus. J. Plant. Biol. 46 (2):90–94; 2003.
Duncan, D. B. Multiple range and multiple F tests. Biometrics 11:1–42; 1955.
Gamborg, O. L.; Miller, R. A.; Ojina, K. Nutritional requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–258; 1968.
Hilliou, F.; Chiristou, P.; Leech, M. J. Development of efficient transformation system for Catharanthus roseus cell cultures using particle bombardment. Plant Sci. 140:179–188; 1999.
Hong, S. B.; Peebles, C.; Shanks, J. V.; San, K. Y.; Gibson, S. I. Terpenoid indole alkaloid production by Catharanthus roseus hairy roots induced by Agrobacterium tumefaciens harboring rol ABC genes. Biotechnol. Bioeng. 93(2):386–390; 2006.
Kaur, K.; Lodha, P.; Kant, U. In vitro regeneration of mosaic virus free Catharanthus roseus (L.) G. Don. plants through callus culture. J. Phytol. Res. 9(1):25–28; 1996.
Kim, S. W.; In, D. S.; Choi, P. S.; Liu, J. R. Plant regeneration from immature zygotic embryo-derived embryogenic calluses and cell suspension cultures of Catharanthus roseus. Plant Cell Tiss. Org. Cult. 76:131–135; 2004.
Kim, S. W.; Jung, K. H.; Song, N. H.; Kwak, S. S.; Liu, J. R. High frequency plant regeneration from anther-derived cell suspension cultures via somatic embryogenesis in Catharanthus roseus. Plant Cell Rep. 13:319–322; 1994.
Lee, S. Y.; Choi, P. S.; Chung, H. J.; In, D. S.; Choi, D. W.; Liu, J. R. Comparison of adventitious shoot formation in petiole explant cultures of 20 cultivars of Catharanthus rosesus. J. Plant Biotechnol. 5(1):59–61; 2003.
Leslie, V. D. F.; Memelink, J. ORCA3, a jasmoate-responsive transcriptional regulator of plant primary and secondary metabolism. Science 289:295–297; 2000.
Liu, C. Z.; Murch, S. J.; EL-Demerdash, M.; Saxena, P. K. Regeneration of the Egyptian medicinal plant Artemisia judaica L. Plant Cell Rep. 21:525–530; 2003.
Lounasmaa, M.; Galambos, J. Indole alkaloid production in Catharanthus roseus cell suspension cultures. Fortschr Chem. Org. Naturst. 55:89–115; 1989.
Mithila, J.; Hall, J. C.; Victor, J. M. R.; Saxena, P. K. Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl.). Plant Cell Rep. 21:408–414; 2003.
Mollers, C.; Sarkar, S. Regeneration of healthy plants from Catharanthus roseus infected with mycoplasma-like organisms through callus culture. Plant Sci. 60:83–89; 1989.
Murashige, I.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Murthy, B. N. S.; Murch, S. J.; Saxena, P. K. Thidiazuron-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): endogenous growth regulator levels and significance of cotyledons. Physiol. Plant. 94:268–276; 1995.
Murthy, B. N. S.; Murch, S.J.; Saxena, P. K. Thidiazuron: a potential regulator of in vitro plant morphogenesis. In Vitro Cell. Dev. Biol.-Plant 34:267–275; 1998.
Piovan, A.; Filippini, R.; Caniato, R.; Dalla vecchia, F.; Innocenti, G.; Cappelletti, E. M.; Puricelli, L. Somatic embryogenesis and indole alkaloid production in Catharanthus roseus. Plant Biosys. 134 (2):179–184; 2000.
Van der Fits, L.; Memelink, J. Comparison of the activities of CaMV 35S and FMV 34S promoter derivatives in Catharanthus roseus cells transiently and stably transformed by particle bombardment. Plant Mol. Biol. 33:943–946; 1997.
Verpoorte, R.; Van der Heijden, R.; Schripsema, J.; Hoge, J. H. C.; Ten Hoppen, H. J. G. Plant cell biotechnology for the production of indole alkaloids: present status and prospects. J Natl. Prod. 56:186–207; 1993.
Zárate, R.; Memelink, J.; Van der Heijden, R.; Verpoorte, R. Genetic transformation via particle bombardment of Catharanthus roseus plants through adventitious organogenesis of buds. Biotechnol. Lett. 21:997–1002; 1999.
Acknowledgement
The authors wish to thank Dr. J. R. Liu (KRIBB, South Korea) for his useful suggestions during this study and providing his published off-prints.
Author information
Authors and Affiliations
Corresponding author
Additional information
Editor: Anna Pretova
Rights and permissions
About this article
Cite this article
Dhandapani, M., Kim, D.H. & Hong, SB. Efficient plant regeneration via somatic embryogenesis and organogenesis from the explants of Catharanthus roseus . In Vitro Cell.Dev.Biol.-Plant 44, 18–25 (2008). https://doi.org/10.1007/s11627-007-9094-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11627-007-9094-x