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Optimization of Renealmia mexicana (Klotzsch ex. Petersen) cultivation in vitro

  • Micropropagation
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Abstract

Renealmia mexicana (Klotzsch ex. Petersen) is a tropical plant found in southern México with an ornamental value and a potential source of curcuminoids. Its distribution in Chiapas has decreased because of deforestation and low propagation and germination rate, so a protocol for in vitro propagation was developed. An orthogonal experimental design of L9 (34) in triplicate was used to investigate the effect of 6-benzyl adenine (BA), indole butyric acid (IBA), silver nitrate (AgNO3), and sucrose on shoot, root, and leaf development of plantlets grown in vitro. Plantlets with well-developed shoots and roots were transferred to pots containing a mixture of peat moss and agrolite for hardening before transfer to soil. The Murashige and Skoog (Physiol. Plant. 15:473–497, 1962) mineral medium (MS) supplemented with 4.4 μM BA, 2.5 μM IBA, 11.7 μM AgNO3y and 5.5% (w/v) sucrose gave most shoots, 8.9 μM BA, 2.5 μM IBA, 17.7 μM AgNO3 and 5.5% (w/v) sucrose most roots, and 8.9 μM BA, 4.9 μM IBA, 11.7 μM AgNO3 and 3.0% (w/v) sucrose most leaves, although other combinations were statistically equivalent in each case. Sucrose was the factor that most explained the variation in the promotion of shoots, roots, and leaves. The protocol developed resulted in up to 100% survival when plantlets were transferred to soil using AgNO3, confirming that hardening of plantlets in vitro using hormonal stimulation was a suitable strategy to improve acclimatization.

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Acknowledgment

A. Domínguez-Estudillo received grant-aided support from Consejo del Sistema Nacional de Educación Tecnológica (COSNET), México. The investigation was done at the laboratory of Plant Biotechnology within the framework of a project to promote tropical agriculture by the agricultural secretary of the State of Chiapas (Mexico).

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Authors and Affiliations

  1. Laboratorio de Biotecnología Vegetal, Instituto Tecnológico de Tuxtla-Gutiérrez, Tuxtla-Gutiérrez, Mexico

    Federico Antonio Gutiérrez Miceli, Adan Domínguez Estudillo, Miguel Abud Archila, Teresa del Rosario Ayora Talavera & Luc Dendooven

  2. Biotechnology and Bioengineering, Cinvestav, Av. I.P.N. 2508, Mexico City, DF, 07360, Mexico

    Luc Dendooven

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  1. Federico Antonio Gutiérrez Miceli
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  2. Adan Domínguez Estudillo
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  3. Miguel Abud Archila
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  4. Teresa del Rosario Ayora Talavera
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  5. Luc Dendooven
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Correspondence to Luc Dendooven.

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Editor: S.J. Murch

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Gutiérrez Miceli, F.A., Estudillo, A.D., Abud Archila, M. et al. Optimization of Renealmia mexicana (Klotzsch ex. Petersen) cultivation in vitro . In Vitro Cell.Dev.Biol.-Plant 44, 33–39 (2008). https://doi.org/10.1007/s11627-007-9079-9

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