Abstract
A three-stage procedure for embryogenesis in Trachyspermum ammi was developed from cotyledon and cotyledonary node explants cultured in Murashige and Skoog (MS) liquid medium supplemented with 0.2 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D). Globular somatic embryos without intervening callus phase developed in 4 wk. The development of embryos to heart and torpedo stages required second-stage subculture of the explants (along with developing embryos) in liquid medium with lower concentrations of 2,4-D. Further development of embryos required a third-stage subculture in hormone-free liquid medium supplemented with 100 mg l−1 casein hydrolysate. Regeneration of complete plantlets occurred after the fully developed somatic embryos were transferred to solidified half-strength MS medium supplemented with 1 mg l−1 gibberellic acid.
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Acknowledgement
S. Purohit gratefully acknowledges Dr. S. Malhotra from National Research Centre on Seed Spices, Ajmer, Rajasthan, for providing seed material.
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Editor: W. Y. Soh
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Purohit, S., Kothari, S.L. Direct somatic embryogenesis from cotyledon and cotyledonary node explants in bishop’s weed Trachyspermum ammi (L.) sprague. In Vitro Cell.Dev.Biol.-Plant 43, 154–158 (2007). https://doi.org/10.1007/s11627-007-9039-4
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DOI: https://doi.org/10.1007/s11627-007-9039-4