Abstract
This study investigated the factors affecting in vitro flowering of Perilla frutescens. The shoots regenerated from cotyledonary and hypocotyl explants cultured on Murashige and Skoog (MS) medium supplemented with benzyladenine (BA) and indole-3-acetic acid, each at 0.5 mg l−1, were excised and transferred to MS medium containing 30 g l−1 of sucrose, 8.25 g l−1 of ammonium nitrate, and 1.0 mg l−1 of BA. After 40 d of culture, 86.2% of shoots flowered and most of which self-fertilized in vitro and produced mature fruits with viable seeds. These seeds were germinated and plants were grown to maturity and flowered in soil under greenhouse conditions. The in vitro flowering system reported in this study may facilitate rapid breeding of P. frutescens and offers a model system for studying the physiological mechanism of flowering.
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Al-Wareh, H.; Trolinder, N. L.; Goodin, J. R. In vitro flowering of potato. HortScience 24: 827–829; 1989.
Avenido, R. A.; Haulea, D. M. In vitro organogenesis and flowering in mungbean (Vigna radiata L.). Phil. J. Crop Sci. 15: 169–173; 1990.
Deaton, M. H.; Buxton J. W.; Hamilton-Kemp, T. R. The effects of growth regulators on development of Nicotiana affinis flowers in vitro. HortScience 19: 509–511; 1984.
Franklin, G.; Pius, P. K.; Ignacimuthul S. Factors affecting in vitro flowering and fruiting of green pea (Pisum sativum L.). Euphytica 115: 65–73; 2000.
Heylen, C.; Vendrig, J. C. The influence of different cytokinins and auxins on flower neoformation in thin cell layers of Nicotiana tabacum L. Plant Cell Physiol. 29: 665–671; 1988.
Hou, S. W.; Jia, J. F. In vitro regeneration of Perilla frutescens from hypocotyl and cotyledon explants. Biol. Plant. 49: 129–132; 2005.
Ignacimuthu, S.; Franklin, G.; Melchias, G. Multiple shoot formation and in vitro fruiting of Vigna mungo (L.) Hepper. Curr. Sci. 73: 733–735; 1997.
Joshi, M.; Nadgauda, R. S. Cytokinins and in vitro induction of flowering in bamboo: Bambusa arundinacea (Retz.) Willd. Curr. Sci. 73: 523–526; 1997.
Kim, K. H.; Lee, Y. H.; Kim, D.; Park, Y. H.; Lee, J. Y.; Hang, Y. S.; Kim, Y. H. Agrobacterium-mediated genetic transformation of Perilla frutescens. Plant Cell Rep. 23:386–390; 2004.
Koezuka Y.; Honda, G.; Tabata M. An intestinal propulsion promoting substance from Perilla frutescens and its mechanism of action. Planta Med. 6: 480–482; 1985.
Lee, B. K.; Yu, S. H.; Kim, Y. H.; Ahn, B. O.; Hur, H. S.; Lee, S. C.; Zhang, Z. Y.; Lee, J. Y. Agrobacterium-mediated transformation of Perilla (Perilla frutescens). Plant Cell Tissue Organ Cult. 83: 51–58; 2005.
Lee, H. S.; Yang, S. G.; Liu, J. R.; Lee, K. W. The control of in vitro flowering of ginseng by growth regulators. Kor. J. Bot. 32: 255–264; 1989.
Makino, T.; Ono, T.; Muso, E. Effect of Perilla frutescens on nitric oxide production and DNA synthesis in cultured murine vascular smooth muscle cells. Phytother. Res. 16: 19–23; 2002.
McCourt, P. Genetic analysis of hormone signaling. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50: 219–243; 1999.
Rastogi, R.; Sawhney, V. K. The role of plant growth regulators, sucrose and pH in the development of floral buds of tomato (Lycopersicon esculentum Mill.) cultured in vitro. J. Plant Physiol. 128: 285–295; 1987.
Saito, K.; Yamazaki, Y. Biochemistry and molecular biology of the late-stage of biosynthesis of anthocyanin: lessons from Perilla frutescens as a model plant. New Phytol. 155: 9–23; 2002.
Shin, H. S.; Kim, S. W. Lipid composition of perilla seed. J. Am. Oil Chem. Soc. 71: 619–622; 1994.
Takeda, H.; Tsuji, M.; Matsumiya, T. Identification of rosmarinic acid as a novel antidepressive substance in the leaves of Perilla frutescens Britton var. acuta Kudo (Perillae Herba). Jpn. J. Psychopharmacol. 22: 15–22; 2002.
Wang, S.; Tang, L.; Chen, F. In vitro flowering of bitter melon. Plant Cell Rep. 20: 393–397; 2001.
Zhang, T.; Cao, Z. Y.; Wang, X. Y. Plant regeneration in vitro directly from cotyledon and hypocotyl explants of Perilla frutescens and their morphological aspects. Biol. Plant. 49: 423–426; 2005.
Zhong, H.; Srinivasan, C.; Sticklen, M. B. In vitro morphogenesis of corn (Zea mays L.). Planta 187: 490–497; 1992.
Zhou, J. H.; Zhou, J. R.; Lin, B. C.; Din, J. Influences of 6-BA and amino acids on flower formation from cotyledons of cucumber in vitro. Plant Physiol. Comm. 6: 31–33; 2004.
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Zhang, T. In vitro flowering of Perilla frutescens . In Vitro Cell.Dev.Biol.-Plant 43, 91–94 (2007). https://doi.org/10.1007/s11627-007-9038-5
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DOI: https://doi.org/10.1007/s11627-007-9038-5