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High-frequency direct shoot regeneration and continuous production of rapid-cycling Brassica oleracea in vitro

Summary

An in vitro method was developed for high-frequency shool regeneration and continuous production of rapid-cycling B. oleracea in large numbers. The high regenerative capacity was tissue-dependent. Developmental polarity (apical and basal ends) of the explants appeared to play a regulatory role in shoot morphogenesis in this system. High-frequency shoot regeneration was obtained with N6-benzyladenine or thidiazuron-supplemented media. Delayed and reduced regenerative ability of cultures in air-tight vessels and the dramatic suppression of shoot regeneration in internodal explants by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid implicate a possible involvement of ethylene in shoot morphogenesis in this species. Rotting of regenerated shoots of B. oleracea occurred readily on α-naphthaleneacetic acid-supplemented media. Rooted plantlets were successfully established in soil and developed normal fertile flowers and viable seeds.

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Correspondence to Sanjay Swarup.

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Cheng, PK., Lakshmanan, P. & Swarup, S. High-frequency direct shoot regeneration and continuous production of rapid-cycling Brassica oleracea in vitro . In Vitro Cell.Dev.Biol.-Plant 37, 592–598 (2001). https://doi.org/10.1007/s11627-001-0104-0

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  • DOI: https://doi.org/10.1007/s11627-001-0104-0

Key words

  • rapid-cycling brassica
  • plant development
  • morphogenesis
  • ethylene
  • thidiazuron