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Soybean cell enlargement oscillates with a temperature-compensated period length of CA. 24 min

  • D. James Morré
  • Rhea Pogue
  • Dorothy M. Morré
Article

Summary

Rate of enlargement of epidermal cells from soybean, when measured at intervals of 1 min using a light microscope equipped with a video measurement system, oscillated with a period length of about 24 min. This oscillation parallels the 24-min periodicity observed for the oxidation of NADH by the external plasma membrane NADH oxidase. The increase in length was not only non-linear but intervals of rapid increase in area alternated with intervals of rapid decrease in area. The length of the period was temperature compensated, and was approximately the same when measured at 14, 24 and 34°C even though the rate of cell enlargement varied over this same range of temperatures. These observations represent the first demonstration of an oscillatory growth behavior correlated with a biochemical activity where the period length of both is independent of temperature (temperature compensated) as is the hallmark of clock-related biological phenomena.

Key words

microscopy growth video-microscopy oxidase NADH 

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Copyright information

© Society for In Vitro Biology 2001

Authors and Affiliations

  • D. James Morré
    • 1
  • Rhea Pogue
    • 1
    • 2
  • Dorothy M. Morré
    • 2
  1. 1.Department of Medicinal ChemistryPurdue UniversityWest LafayetteUSA
  2. 2.Department of Foods and NutritionPurdue UniversityWest LafayetteUSA

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