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In Vitro Cellular & Developmental Biology - Plant

, Volume 36, Issue 5, pp 370–373 | Cite as

In vitro differentiation and plant regeneration of Albizia chinesis (Osb.) Merr

  • R. K. Sinha
  • K. Majumdar
  • S. Sinha
Article

Summary

Petiolar and distal cotyledonary segments (PCS and DCS) of Albizia chinensis were cultured on Murashige and Skoog's (MS; 1962) medium and induced to form adventitious shoot buds in the presence of either cytokinins 6-benzylamino purine (BAP), kinetin (KN) or thidiazuron (TDZ). Superiority of BAP in inducing shoot bud and differentiation was observed. PCS was more morphogenic to shoot bud differentiation than DCS. TDZ was highly effective in inducing shoot buds, but arrested shoot growth, while KN produced more callus during differentiation of shoots. Rapid and high rate of shoot multiplication per explant was achieved through subculture in MS medium containing BAP (1.0 mg l−1) and indole-3-acetic acid (IAA) (0.5 mg l−1). BAP at low concentration was required to enhance shoot multiplication and elongation. Successful rooting of regenerated shoots was carried out in a two-step culture procedure in MS media with indole-3-butyric acid (IBA) (2.0 mg l−1) and subsequent subculture in IBA-free medium.

Key words

Albizia chinensis (Osb.) Merr cytokinin in vitro differentiation plant regeneration 

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Copyright information

© Society for In Vitro Biology 2000

Authors and Affiliations

  • R. K. Sinha
    • 1
  • K. Majumdar
    • 1
  • S. Sinha
    • 1
  1. 1.Cytogenetics and Plant Tissue Culture Laboratory, Department of Life SciencesTripura UniversityAgartalaIndia

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