Summary
A protocol is described for rapid multiplication of Piper barberi Gamble (Piperaceae) through shoot tip and nodal explant cultures. Nodal explants with a single axillary meristem showed three times better response with respect to shoot proliferation when compared to shoot tip explants. The best shoot proliferation response of nodal explants was observed with a cytokinin combination of N6-benzyladenine (4.43 μM) and kinetin (2.32 μM), with 88% bud break. The number of shoot initials (2.4) produced per nodal explant was twice the number of shoot initials (1.2) per shoot tip. An average of 6.9±0.58 adventitious shoots were observed from the proximal end of the internodal explants on Mursashige and Skoog (1962) (Ms) basal medium supplemented with N6-benzyladenine (2.22 μM) and kinetin (0.46 μM). A multiplication rate of 82 shoots per explant could be achieved after 9 wk of subculturing. The in vitro shoots were rooted on one-half and one-quarter MS basal medium. The shoots rooted on one-quarter MS in the dark produced eight roots with an average root length of 3.36 cm and 98% survival. These plants were transferred to the field with a survival rate of 75%.
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References
Aminuddin; Johri, J. K.; Anis, M.; Balasubrahmanyam, V. R. Regeneration of Piper betle from callus tissue. Curr. Sci. 65:793–796; 1993.
Anand, A.; Parani, M.; Rao, C. S.; Latha, R.; Balakrishna, P. Micropropagation and genetic fidelity studies in Piper longum. In: Edison, S., Ramana, K. V., Sasikumar, B., Nirmal Babu, Santhosh, J. E., eds. Biotechnology of spices, medicinal and aromatic plants. Calicut: Indian Society for Spices; 1997:94–97.
Babu, N.; Ramakrishna, N. K. R.; Johnson, G. K.; Ravindran, P. N. Piper barberi Gamble—a redescription of the species with a note on the karyotype. J. Spices Aromatic Crops. 1:88–93; 1992.
Babu, N.; Rema, J.; Pillai, S. R. D.; Samsudeen, K.; Ravindran, P. N. Micropropagation of an endangered species of Piper barberi Gamble and its conservation. Indian J. Plant Genet. Resources 9:179–182: 1996.
Bhat, S. R.; Chandel, K. P. S.; Malik, S. K. Plant regeneration from various explants of cultivated Piper species. Plant Cell Rep. 14:398–402; 1995.
Fitchet, M. Establishment of P. nigrum in vitro. Acta Hortic. 275:285–291; 1990.
Johri, J. K.; Aminuddin; Aruna, P. Regeneration of betelvine (Piper betle L.) through somatic embryogenesis. Indian J. Exp. Biol. 34:83–85; 1996.
Lloyd, G.; McCown, B. Commercially feasible micropropagation of mountain laurel Kalmia latifolia by use of shoot tip culture. Int. Plant Prop. Soc. Proc. 30:421–427; 1981.
Mathew, P. J.; Mathew, P. M., Cytological studies on Piper barberi—a rare species endemic to Western Ghats. J. Spices Aromatic Crops 1:81–83; 1992.
Mathews, V. H.; Rao, P. S. In vitro responses in black pepper (Piper nigrum). Curr. Sci. 53:183–186; 1984.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassay with tobacco cultures. Physiol. Plant. 15:473–497; 1962.
Nayar, M. P.; Sastry, A. R. K. Red data book of Indian plants, vol. 2. Calcutta; Botanical Survey of India; 1988:181–182.
Philip, V. J.; Dominic, J.; Triggs, G. S.; Dickinson, N. M. Micropropagation of black pepper (Piper nigrum L.) through shoot tip cultures. Plant Cell Rep. 12:41–44; 1992.
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Anand, A., Srinivasa Rao, C. A rapid in vitro propagation protocol for Piper barberi Gamble, a critically endangered plant. In Vitro Cell.Dev.Biol.-Plant 36, 61–64 (2000). https://doi.org/10.1007/s11627-000-0014-6
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DOI: https://doi.org/10.1007/s11627-000-0014-6