References
Ausubel, F. M.; Brent, R.; Kinston, R. E.; Moore, D. D.; Seidman, J. G.; Smith, J. A.; Struhl, K. In: Current protocols in molecular biology. New York: John Wiley and Sons; 1994;7.3.9.
Green, A. E.; Charney, J. Characterization and identification of insect cell cultures. Curr. Top. Microbiol. Immunol. 55:51–61; 1971.
Kshirsagar, S. G.; Patole, M. S.; Shouche, Y. S. Characterization of insect cell lines: heteroduplex analysis employing a mitochondrial 16S ribosomal RNA gene fragment. Anal. Biochem. 253:65–69; 1997.
Kshirsagar, S. G.; Patole, M. S.; Shouche, Y. S. Insect cell line characterisation by DGGE. In Vitro: In press.
McIntosh, A. H.; Grasela, J. J.; Matteri, R. L. Identification of insect cell lines by DNA amplification fingerprinting (DAF). Insect Mol. Biol. 5:187–195; 1996.
Meade, H. M.; Long, S. R.; Ruvkun, C. B.; Brown, S. E.; Ausubel, F. M. Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis. J. Bacteriol. 149:114–122; 1982.
Olsen, G. J.; Woese, C. R. Ribosomal RNA: key to phylogeny. FASEB J. 7:113–123; 1993.
O’Neil, S. L.; Giordano, R.; Colbert, A. M. E.; Karr, T. L.; Robertson, H. M. 16S rRNA phyoogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc. Natl. Acad. Sci. USA 89:2699–2702; 1992.
Pant, U.; Sudeep, A. B.; Athawale, S. S.; Banerjee, K. A new cell line from larval ovaries of Spodoptera litura (F.) (Lepidoptera: Noctuidae). In Vitro 33:161–163; 1997.
Pant, U.; Athawale, S. S.; Basu, A.; Banerjee, K. A new cell line established from pupal ovaries of Spodoptera litura (F.) (Lepidoptera: Noctuidae). Ind. J. Exp. Biol. 36:195–198; 1998.
Sambrook, J.; Fritsch, E. F.; Maniatis, T.: In: Molecular cloning: a laboratory manual: 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1989;9;16–19.
Tang, J.; Unnaschi, R. R. Heteroduplex analysis in medical entomology: a rapid and sensitive sequence based tool for population and phylogenetic studies. Parasitol. Today 13:271–274; 1997.
Vaughn, J. L.; Goodwin, R. G.; Tompkins, G. J.; McCawley, P. The establishment of two insect cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae) In Vitro 13:213–217; 1977.
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Shouche, Y.S., Patole, M.S., Pant, U. et al. Authentication of two cell lines developed from the larval and pupal ovaries of Spodoptera litura by rRNA based methods. In Vitro Cell.Dev.Biol.-Animal 35, 244–245 (1999). https://doi.org/10.1007/s11626-999-0066-9
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DOI: https://doi.org/10.1007/s11626-999-0066-9