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Initial aspects of mineralization at the dentino-enamel junction in embryonic mouse incisor in vivo and in vitro: A tem comparative study

  • Growth, Differentiation And Senescence
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Summary

The frontier between the enamel organ and the dental papilla, the future dentino-enamel junction, undergoes coordinated modifications. The mineralization of the extracellular matrix starts within the predentine, which is a prerequisite for the formation of the first enamel crystallites in vivo. We investigated the dentino-enamel junction using the embryonic mouse incisor as a model. Our data showed that the notion of the dentino-enamel junction should not be restricted to the thin interface classically described. A temporo-spatial survey from the epithelio-mesenchymal junction to the dentino-enamel junction delineated a clear sequence of events characterized by the early deposition of electron-dense granules, followed by the appearance of patches of stippled material at the dentino-enamel junction. The first tiny enamel crystallites appeared in the vicinity of this material which presented a well-ordered alignment. The comparison of data obtained in vivo on 17-, 18-, 19-d-old embryonic incisors with those obtained in vitro using 15-d-old embryonic incisors cultured for 7 d emphasizes the relevance of this sequence. Helicoidal growing crystals were observed in cultured tooth germs but never in vivo.

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  1. Institut de Biologie Médicale—INSERM U 424 Faculté de Médecine, 11 rue Humann, 67085, Strasbourg Cedex, France

    J. M. Meyer, P. Bodier-Houllé, F. J. G. Cuisinier, H. Lesot & J. V. Ruch

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  1. J. M. Meyer
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  2. P. Bodier-Houllé
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  3. F. J. G. Cuisinier
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  4. H. Lesot
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  5. J. V. Ruch
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Meyer, J.M., Bodier-Houllé, P., Cuisinier, F.J.G. et al. Initial aspects of mineralization at the dentino-enamel junction in embryonic mouse incisor in vivo and in vitro: A tem comparative study. In Vitro Cell.Dev.Biol.-Animal 35, 159–168 (1999). https://doi.org/10.1007/s11626-999-0019-3

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  • DOI: https://doi.org/10.1007/s11626-999-0019-3

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