Skip to main content

Advertisement

Log in

Use of flow cytometry to study growth of human airway smooth muscle cells

  • Letter To The Editor
  • Scientific Reports
  • Published:
In Vitro Cellular & Developmental Biology - Animal Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Caplice, N. M.; West, M. J.; Campbell, G. R., et al. Inhibition of human vascular smooth muscle cell growth by heparin. Lancet 344:97–98; 1994.

    Article  PubMed  CAS  Google Scholar 

  2. Drach, J. C.; Thomas, M. A.; Barnett, J. W., et al. Tritiated thymidine incorporation does not measure DNA synthesis in ribavirin-treated cells. Science 212:549–551; 1981.

    Article  PubMed  CAS  Google Scholar 

  3. Grainger, D. J.; Witchell, C. M.; Watson, J. V., et al. Heparin decreases the rate of proliferation of rat vascular smooth muscle cells by releasing transforming growth factor a-like activity from serum. Cardiovasc. Res. 27:2238–2247; 1993.

    PubMed  CAS  Google Scholar 

  4. Gray, J. W.; Dolbeare, F.; Pallavicini, M. G., et al. Cell cycle analysis using flow cytometry. Int. J. Radiat. Biol. 49:237–255; 1986.

    CAS  Google Scholar 

  5. Hadrava, V.; Tremblay, J.; Hamet, P. Intrinsic factors involved in vascular smooth muscle cell proliferation in hypertension. Clin. Invest. Med. 14:535–544; 1991.

    PubMed  CAS  Google Scholar 

  6. Hadrava, V.; Tremblay, J.; Sekaly, R.-P., et al. Accelerated entry of aortic smooth muscle cells from spontaneously hypertensive rats into the S phase of the cell cycle. Biochem. Cell Biol. 70:599–604; 1991.

    Article  Google Scholar 

  7. Hirst, S. J.; Barnes, P. J.; Twort, C. H. C. Quantifying proliferation of cultured human and rabbit airway smooth muscle cells in response to serum and platelet-derived growth factor. Am. J. Respir. Cell Mol. Biol. 7:574–581; 1992.

    PubMed  CAS  Google Scholar 

  8. Kondo, T.; Konishi, F.; Inui, H., et al. Differing signal transductions elicited by three isoforms of platelet-derived growth factor in vascular smooth muscle cells. J. Biol. Chem. 268:4458–4464; 1993.

    PubMed  CAS  Google Scholar 

  9. Larsen, J. K.; Munch-Petersen, B.; Christiansen, J., et al. Flow cytometric discrimination of mitotic cells: resolution of M, as well as G1, S, and G2 phase nuclei with mithramycin, propidium iodide, and ethidium bromide after fixation with formaldehyde. Cytometry 7:54–63; 1986.

    Article  PubMed  CAS  Google Scholar 

  10. Murat, J. C.; Gamet, L.; Cazenave, Y., et al. Questions about the use of [3H]thymidine incorporation as a reliable method to estimate cell proliferation rate. Biochem. J. 270:563–564; 1990.

    PubMed  CAS  Google Scholar 

  11. Oliver, M. H.; Harrison, N. K.; Bishop, J. E., et al. A rapid and convenient assay for counting cells cultured in microwell plates: application for assessment of growth factors. J. Cell Sci. 92:513–518; 1989.

    PubMed  Google Scholar 

  12. Tachibana, M.; Deguchi, N.; Jitsukawa, S., et al. Quantification of cell kinetic characteristics using flow cytometric measurements of deoxyribonucleic acid and bromodeoxyuridine for bladder cancer. J. Urol. 145:963–967; 1991.

    PubMed  CAS  Google Scholar 

  13. Yamamoto, M.; Yamamoto, K. Growth regulation in primary culture of rabbit arterial smooth muscle cells by platelet-derived growth factor, insulin-like growth factor-1, and epidermal growth factor. Exp. Cell Res. 212:62–68; 1994.

    Article  PubMed  CAS  Google Scholar 

  14. Zhang, Z. D.; Cox, G. MTT assay overestimates human airway smooth muscle cell number in culture. Biochem. Mol. Biol. Int. 38:431–436; 1996.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, ZD., Cox, G. Use of flow cytometry to study growth of human airway smooth muscle cells. In Vitro Cell.Dev.Biol.-Animal 34, 275–277 (1998). https://doi.org/10.1007/s11626-998-0001-5

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11626-998-0001-5

Keywords

Navigation