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In vitro angiogenesis is modulated by the mechanical properties of fibrin gels and is related to αvβ3 integrin localization

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Summary

This study deals with the role of the mechanical properties of matrices in in vitro angiogenesis. The ability of rigid fibrinogen matrices with fibrin gels to promote capillarylike structures was compared. The role of the mechanical properties of the fibrin gels was assessed by varying concentration of the fibrin gels. When the concentration of fibrin gels was decreased from 2 mg/ml to 0.5 mg/ml, the capillarylike network increased. On rigid fibrinogen matrices, capillarylike structures were not formed. The extent of the capillarylike network formed on fibrin gels having the lowest concentration depended on the number of cells seeded. The dynamic analysis of capillarylike network formation permitted a direct visualization of a progressive stretching of the 0.5 mg/ml fibrin gels. This stretching was not observed when fibrin concentration increases. This analysis shows that 10 h after seeding, a prearrangement of cells into ringlike structures was observed. These ringlike structures grew in size. Between 16 and 24 h after seeding, the capillarylike structures were formed at the junction of two ringlike structures. Analysis of the αvβ3 integrin localization demonstrates that cell adhesion to fibrinogen is mediated through the αvβ3 integrin localized into adhesion plaques. Conversely, cell adhesion to fibrin shows a diffuse and dot-contact distribution. We suggest that the balance of the stresses between the tractions exerted by the cells and the resistance of the fibrin gels triggers an angiogenic signal into the intracellular compartment. This signal could be associated with modification in the αvβ3 integrin distribution.

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Authors and Affiliations

  1. Laboratoire de Bioénergétique Fondamentale et Appliquée, Université Joseph Fourier, B.P.53, 38041, Grenoble Cedex 9, France

    Bruno Vailhé & Léone Tranqui

  2. Laboratoire Dynamique de l’Organisation des Génomes, Institut Albert Bonniot, Université Joseph Fourier, 38706, La Tronche Cedex, France

    Yves Usson

  3. Laboratoire TIMC, UMR CNRS 5525, Faculté de Médecine, Institut Albert Bonniot, Domaine de la Merci, 38700, La Tronche, France

    Philippe Tracqui

  4. Laboratoire de Neurobiologie du Développement, E.P.H.E., 34095, Montpellier Cedex 05, France

    Xavier Ronot

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  1. Bruno Vailhé
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Vailhé, B., Ronot, X., Tracqui, P. et al. In vitro angiogenesis is modulated by the mechanical properties of fibrin gels and is related to αvβ3 integrin localization. In Vitro Cell.Dev.Biol.-Animal 33, 763–773 (1997). https://doi.org/10.1007/s11626-997-0155-6

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  • DOI: https://doi.org/10.1007/s11626-997-0155-6

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