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Establishment and immunocharacterization of an immortalized pancreatic cell line derived from the H-2Kb-tsA58 transgenic mouse

  • Growth, Differentiation And Senescence
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Summary

This study describes the establishment and characterization of an immortalized cell line derived from the pancreas of an adult H-2Kb-tsA58 transgenic mouse. These cells, designated IMPAN for IMmortalized PANcreatic cells, displayed a cobblestone appearance typical of confluent epithelial cells and a distinct polarity in the organization of their cytoplasmic organelles. Immunocytochemical studies revealed that all IMPAN cells stained positively for a wide range of markers characteristic of pancreatic acinar cells, namely the secretory products α-amylase, chymotrypsinogen, DNAse, the lectinlike secretory protein PAP (pancreatitis associated protein), and the zymogen granule membrane proteins GP-2 and gp300. They also stained positively for carbonic anhydrase II and cytokeratin 19, two proteins characteristic of pancreatic duct cells, as well as for rab3A, a small GTP-binding protein specifically localized in pancreatic islet cells. No reactivity was ever obtained with insulin antibodies. Taken together, these results show that the IMPAN cells exhibit a phenotype comparable to exocrine pancreatic acinar cells. However the expression of some proteins more specific to duct and islet cells make them similar to in vivo or in vitro growing acinar cells. The cell line should be a valuable model to study the mechanisms of growth, differentiation, and transformation of the exocrine pancreatic acinar cell.

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Authors and Affiliations

  1. Département de biologie, Faculté des Sciences, Université de Sherbrooke, 2500, Boulevard Université, J1K 2R1, Sherbrooke, Québec, Canada

    Richard Blouin, Gilles Grondin, Jude Beaudoin, Nathalie Daigle, Brian G. Talbot & Denis Lebel

  2. Département de médecine, Faculté de Médecine, Université de Sherbrooke, 2500, Boulevard Université, J1K 2R1, Sherbrooke, Québec, Canada

    Yoshi Arita & Jean Morisset

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  1. Richard Blouin
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  2. Gilles Grondin
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Blouin, R., Grondin, G., Beaudoin, J. et al. Establishment and immunocharacterization of an immortalized pancreatic cell line derived from the H-2Kb-tsA58 transgenic mouse. In Vitro Cell.Dev.Biol.-Animal 33, 717–726 (1997). https://doi.org/10.1007/s11626-997-0130-2

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  • DOI: https://doi.org/10.1007/s11626-997-0130-2

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