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Multiplication of a granulosis virus isolated from the potato tuber moth in a new established cell line of Phthorimaea operculella

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Summary

A newly established cell line was obtained from the culture of embryonic cells of the potato tuber moth Phthorimaea operculella in low temperature conditions (19° C) using modified Grace’s medium supplemented with 10% fetal bovine serum. The population doubling time was about 80 h when cells were cultivated at 19°C and 38 h at 27° C. The cell line had a relatively homogeneous population consisting of various sized spherical cells. The cells were cultivated for more than 25 passages. Their polypeptidic profile was different from profiles of other P. operculella cell lines we previously described and from other lepidopteran cells. The new cell line was designated ORS-Pop-95.

The complete replication of the potato tuber moth granulosis virus (PTM GV) was obtained in vitro by both viral infection and DNA transfection. PTM GV multiplied at a significant level during several passages of the cell line that was maintained at 19° C. As long as the cells were maintained at 19° C, virus multiplication could also be obtained at the same rate at 27° C. To compare PTM GV multiplied both in vivo and in vitro, we used morphological identification, serological, DNA probe diagnosis and endonuclease digest profile analysis and confirmed the identity of the virus.

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References

  1. Burand, J. P.; Summers, M. D.; Smith, G. E. Transfection with baculovirus DNA. Virology 101:286–290; 1980.

    Article  CAS  PubMed  Google Scholar 

  2. Chang, P. M.; Tanada, Y. Serological study on the transmission of a granulosis virus of the armyworm, Pseudaletia unipuncta (Lepidoptera: Noctuidae), to other lepidopterous species. J. Invertebr. Pathol. 31:106–117; 1978.

    Article  Google Scholar 

  3. Chen, Q.; Dai, X. Replication of Pieris rapae granulosis virus in an insect cell line. In: Kuroda, Y.; Kurstak, E.; Maramorosch, K., ed. Invertebrate and fish tissue culture; 1987.

  4. Cochran, M. A.; Carstens, E. B.; Eaton, B. T., et al. Molecular cloning and physical mapping of restriction endonuclease fragments of Autographa californica nuclear polyhedrosis virus DNA. J. Virol. 41:940–948; 1982.

    PubMed  CAS  Google Scholar 

  5. Crook, N. E. Baculoviridae: subgroup B. In: Kurstak, E., ed. Viruses of invertebrates. New York: M. Dekker, Inc.; 1991:73–110.

    Google Scholar 

  6. Das, G. P. L.; Magallona, E. D.; Raman, K. V., et al. Effects of different components of IPM in the management of the potato tuber moth, in storage. Agric. Ecol. Environm. 41:321–325; 1992.

    Article  Google Scholar 

  7. Dwyer, K. G.; Webb, S. E.; Shelton, A. M. Establishment of cell lines from Pieris rapae embryos. Characterization and susceptibility to baculoviruses. J. Invertebr. Pathol. 52:268–274; 1988.

    Article  Google Scholar 

  8. Goodwin, R. H.; Vaughn, J. L.; Adams, J. R., et al. Replication of a nuclear polyhedrosis virus in an established cell line. J. Invertebr. Pathol. 16:284–288; 1970.

    Article  PubMed  CAS  Google Scholar 

  9. Granados, R. R.; Derksen, A. C. G.; Dwyer, K. G. Replication of the Trichoplusia ni granulosis virus and nuclear polyhedrosis viruses in cell cultures. Virology 152:472–476; 1986.

    Article  CAS  PubMed  Google Scholar 

  10. Hink, W. F. The 1979 compilation of invertebrate cell lines and culture media. In: Kurstack, E.; Maramorosch, K.; Dübendorfer, A., ed. Invertebrate systems in vitro. Amsterdam: Elsevier; 1980:533–578.

    Google Scholar 

  11. Kelly, D. C.; Edwards, M. L.; Evans, H. F., et al. The use of the enzyme linked immunosorbent assay to detect a nuclear polyhedrosis virus in Heliothis armigera larvae. J. Gen. Virol. 40:465–469; 1978.

    CAS  Google Scholar 

  12. Laemmli, U. K. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature (London) 227:680–685; 1970.

    Article  CAS  Google Scholar 

  13. Léry, X.; El-Tarras, A.; Abol-Ela, S., et al. Yield and activity of Autographa californica nuclear polyhedrosis virus and Phthorimaea operculella granulosis virus in cloned and uncloned cell lines of P. operculella. J. Appl. Entomol. (in press).

  14. Léry, X.; Fédière, G. Effect of different amino acids and vitamins on lepidopteran cell cultures. J. Invertebr. Pathol. 55:47–51; 1990.

    Article  Google Scholar 

  15. Léry, X.; Zeddam, J. L.; Giannotti, J., et al. Establishment of a cell line derived from embryos of the potato tuber moth Phthorimaea operculella (Zeller). In Vitro Cell. Dev. Biol. 31:836–839; 1995.

    Google Scholar 

  16. Miltenburger, H. G.; Naser, W. L.; Harvey, J. P., et al. The cellular substrate: a very important requirement for baculovirus in vitro replication. Z. Naturforsch. 39c:993–1002; 1984.

    CAS  Google Scholar 

  17. Mitsuhashi, J. A continuous cell line from pupal ovaries of the common cutworm Spodoptera litura (Lepidoptera: Noctuidae). Appl. Entomol. Zool. 30:75–82; 1995.

    Google Scholar 

  18. Naser, W. L.; Miltenburger, H. G.; Harvey, J. P., et al. In vitro replication of the Cydia pomonella (codling moth) granulosis virus. FEMS Microbiol. Lett. 24:117–121; 1984.

    Article  Google Scholar 

  19. Ouchterlony, O. Diffusion in gel. Method for immunological analysis. Prog. Allergy 5:1–78; 1958.

    Article  PubMed  CAS  Google Scholar 

  20. Pant, U.; Macarenhas, A. F.; Jagannathan, V. In vitro cultivation of a cell line from embryonic tissue of potato tuber moth Gnorimoschema operculella (Zeller). Indian J. Exp. Biol. 15:244–245; 1977.

    Google Scholar 

  21. Raman, K. V.; Booth, R. H.; Palacios, M. Control of potato tuber moth Phthorimaea operculella (Zeller) in rustic potato stores. Trop. Sci. 27:175–194; 1987.

    Google Scholar 

  22. Reuveny, S.; Kim, Y. J.; Kemp, C. W., et al. Effect of temperature and oxygen on cell growth and recombinant protein production in insect cell cultures. Appl. Microbiol. Biotechnol. 38:619–623; 1993.

    Article  PubMed  CAS  Google Scholar 

  23. Reynolds, E. S. The use of lead citrate at light pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17:208–212; 1963.

    Article  PubMed  CAS  Google Scholar 

  24. Summers, M. D.; Smith, G. E. A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agric. Exp. Sta. Bull. 1555:1–37; 1987.

    Google Scholar 

  25. Tweeten, K. A.; Bulla, L. A.; Consigli, R. A. Applied and molecular aspects of insect granulosis viruses. Microbiol. Rev. 45:379–408; 1981.

    PubMed  CAS  Google Scholar 

  26. Vickers, J. M.; Cory, J. S.; Entwistle, P. F. DNA characterization of eight geographic isolates of granulosis virus from the potato tuber moth (Phthorimaea operculella) (Lepidoptera, Gelechiidae). J. Invertebr. Pathol. 57:334–342; 1991.

    Article  CAS  Google Scholar 

  27. Von Arx, R.; Goveder, J.; Cheikh, M., et al. Integrated control of potato tuber moth Phthorimaea operculella (Zeller). Insect Sci. Applic. 8:989–994; 1987.

    Google Scholar 

  28. Winstanley, D. An improved cell line susceptible to a granulosis virus. In: VIth international colloquium on invertebrate pathology and microbial control. Montpellier, France, 28 August–2 September; 1994.

  29. Winstanley, D.; Crook, N. E. Replication of Cydia pomonella granulosis virus in cell cultures. J. Gen. Virol. 74:1599–1609; 1993.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Entomovirology laboratory ORSTOM, Giza code 12211, B.O. 26, Cairo, Egypt

    Xavier Léry, Joseph Giannotti, Ahmed Taha & Said Abol-Ela

  2. Station de Recherche de Pathologie Comparée, 30380, St Christol-lès-Alès, France

    Marc Ravalec

Authors
  1. Xavier Léry
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  2. Joseph Giannotti
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  3. Ahmed Taha
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  4. Marc Ravalec
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  5. Said Abol-Ela
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Léry, X., Giannotti, J., Taha, A. et al. Multiplication of a granulosis virus isolated from the potato tuber moth in a new established cell line of Phthorimaea operculella . In Vitro Cell.Dev.Biol.-Animal 33, 640–646 (1997). https://doi.org/10.1007/s11626-997-0115-1

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  • DOI: https://doi.org/10.1007/s11626-997-0115-1

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