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3T3-L1 adipocytes as a cell culture model of insulin resistance

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References

  1. Bak, J. F. Insulin receptor function and glycogen synthase activity in human skeletal muscle. Dan. Med. Bull. 41:179–192; 1994.

    PubMed  CAS  Google Scholar 

  2. Czech, M. P.; Clancey, B. M.; Pessino, A., et al. Complex regulation of simple sugar transport in insulin-responsive cells. TIBS 17:197–201; 1992.

    PubMed  CAS  Google Scholar 

  3. Douen, A. G.; Jones, M. N. Insulin processing and signal transduction in rat adipocytes. Biochim. Biophys. Acta 1010:363–368; 1989.

    Article  PubMed  CAS  Google Scholar 

  4. Häring, H. U. The insulin receptor: signaling mechanism and contribution to the pathogenesis of insulin resistance. Diabetologia 34:848–861; 1991.

    Article  PubMed  Google Scholar 

  5. Harrison, S. A.; Buxton, J. M.; Clancy, B. M., et al. Insulin regulation of hexose transport in mouse 3T3-L1 cells expressing the human HepG2 glucose transporter. J. Biol. Chem. 265:20106–20116; 1990.

    PubMed  CAS  Google Scholar 

  6. Hausdorff, S. F.; Frangioni, J. V.; Birnbaum, M. J. Role of p21ras in insulin-stimulated glucose transport in 3T3-L1 adipocytes. J. Biol Chem. 269:21391–21394; 1994.

    PubMed  CAS  Google Scholar 

  7. Hess, S. L.; Suchin, C. R.; Saltiel, A. R. The specific protein phosphatase inhibitor okadaic acid differentially modulates insulin action. J. Cell. Biochem. 45:374–380; 1991.

    Article  PubMed  CAS  Google Scholar 

  8. James, D. E.; Piper, R. C. Insulin resistance, diabetes and the insulin-regulated trafficking of GLUT-4. J. Cell Biol. 126:1123–1126; 1994.

    Article  PubMed  CAS  Google Scholar 

  9. Kahn, C. R. Insulin action, diabetogenes, and the cause of Type II diabetes. Diabetes 43:1066–1084; 1994.

    Article  PubMed  CAS  Google Scholar 

  10. Karlsson, F. A.; Grunfeld, C.; Kahn, C. R., et al. Regulation of insulin receptors and insulin responsiveness in 3T3-L1 fatty fibroblasts. Endocrinology 104:1383–1392; 1979.

    Article  PubMed  CAS  Google Scholar 

  11. Knutson, V. P. The acute and chronic effects of glucocorticoids on insulin receptor and insulin responsiveness: transient fluctuations in intracellular receptor level parallel transient fluctuations in responsiveness. J. Biol. Chem. 261:10306–10312; 1986.

    PubMed  CAS  Google Scholar 

  12. Knutson, V. P.; Ronnett, G. V.; Lane, M. D. Rapid, reversible internalization of cell surface insulin receptors: correlation with insulin-induced down-regulation. J. Biol. Chem. 258:12139–12142; 1983.

    PubMed  CAS  Google Scholar 

  13. Lin, T.-A.; Lawrence, J. C., Jr. Activation of ribosomal protein S6 kinases does not increase glycogen synthesis or glucose transport in rat adipocytes. J. Biol. Chem. 269:21255–21261; 1994.

    PubMed  CAS  Google Scholar 

  14. Morales, L. M.; Campos, G.; Ryder, E., et al. Insulin and lipogenesis in rat adipocytes. I. Effect of insulin incubation on lipid synthesis by isolated rat adipocytes. Biochem. Biophys. Res. Comm. 188:807–812; 1992.

    Article  PubMed  CAS  Google Scholar 

  15. Morales, L. M.; Campos, G.; Ryder, E., et al. Insulin and lipogenesis in rat adipocytes. II. Effect of high levels of insulin and glucose on lipid synthesis in isolated rat adipocytes. Biochem. Biophys. Res. Comm. 199:1151–1157; 1994.

    Article  PubMed  CAS  Google Scholar 

  16. Myers, M. G.; Backer, J. M.; Siddle, K., et al. The insulin receptor functions normally in Chinese hamster ovary cells after truncation of the C terminus. J. Biol. Chem. 266:10616–10623; 1991.

    PubMed  CAS  Google Scholar 

  17. Pederson, O.; Kahn, C. R.; Kahn, B. B. Divergent regulation of the Glut 1 and Glut 4 glucose transporters in isolated adipocytes from Zucker rats. J. Clin. Invest. 89:1964–1973; 1992.

    Google Scholar 

  18. Radin, M. S. Preparation of lipid extracts. Methods Enzymol. 14:245–254; 1969.

    Article  CAS  Google Scholar 

  19. Robinson, K. A.; Sens, D. A.; Buse, M. G. Pre-exposure to glucosamine induces insulin resistance to glucose transport and glycogen synthesis in isolated rat skeletal muscles. Study of mechanisms in muscle and in rat-1 fibroblasts overexpressing the human insulin receptor. Diabetes 42:1333–1346; 1993.

    Article  PubMed  CAS  Google Scholar 

  20. Ronnett, G. V.; Knutson, V. P.; Lane, M. D. Insulin-induced down-regulation of insulin receptors in 3T3-L1 adipocytes: altered rate of receptor inactivation. J. Biol. Chem. 257:4285–4291; 1982.

    PubMed  CAS  Google Scholar 

  21. Ronnett, G. V.; Tennekoon, G.; Knutson, V. P., et al. Kinetics of insulin receptor transit to and removal from the plasma membrane: effect of insulin-induced down-regulation in 3T3-L1 adipocytes. J. Biol. Chem. 258:283–290; 1983.

    PubMed  CAS  Google Scholar 

  22. Sowers, J. R. Insulin resistance, hyperinsulinemia, dyslipidemia, hypertension, and accelerated atherosclerosis. J. Clin. Pharmacol. 32:529–535; 1992.

    Article  PubMed  CAS  Google Scholar 

  23. Tarlow, D. M.; Watkins, P. A.; Reed, R. E., et al. Lipogenesis and the synthesis and secretion of very low density lipoproteins by avian liver cells in nonproliferating monolayer culture. J. Cell Biol. 73:332–353; 1977.

    Article  PubMed  CAS  Google Scholar 

  24. Traxinger, R. R.; Marshall, S. Recovery of maximal insulin responsiveness and insulin sensitivity after induction of insulin resistance in primary cultured adipocytes. J. Biol. Chem. 264:8156–8163; 1989.

    PubMed  CAS  Google Scholar 

  25. Weiland, M.; Brandenburg, C.; Brandenburg, D., et al. Antagonistic effects of a covalently dimerized insulin derivative on insulin receptors in 3T3-L1 adipocytes. Proc. Natl. Acad. Sci. USA 87:1154–1158; 1990.

    Article  PubMed  CAS  Google Scholar 

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  1. Department of Pharmacology, The University of Texas-Houston Medical School, 6431 Fannin, 77030, Houston, Texas

    Victoria P. Knutson & Yvonne Balba

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  1. Victoria P. Knutson
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  2. Yvonne Balba
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Knutson, V.P., Balba, Y. 3T3-L1 adipocytes as a cell culture model of insulin resistance. In Vitro Cell.Dev.Biol.-Animal 33, 77–81 (1997). https://doi.org/10.1007/s11626-997-0025-2

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