Abstract
The purpose of this research was to investigate whether or not avicularin (AVL) possesses any anticancer properties when tested against lung cancer. In the beginning, the effect that it had on the cellular viability of A549 cells was investigated, and it was discovered that AVL has a considerable negative impact on cellular viability. Following that, an investigation using flow cytometry was carried out to investigate its function in the process of apoptosis and the cell cycle of A549 cells. It has been discovered that AVL significantly promotes apoptosis and stops the cell cycle at the G2/M phase. The colony-forming capacity of A549 cells was observed to be greatly suppressed as the AVL concentration increased compared to the group that received no treatment. In addition to this, the benzo(a)pyrene in vivo model was established in order to investigate the pharmacological value of AVL. The findings revealed that AVL greatly prevented the formation of pro-inflammatory cytokines, in addition to the reduction in oxidative stress, which was evidenced by a reduction in the concentration of TNF-α, IL-1β, IL-6, and MDA with an improvement in the concentration of SOD and GPx, respectively. Our results successfully demonstrated the pharmacological benefit of avicularin against lung cancer, and it has been suggested that it showed a multifactorial effect.
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Data Availability
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Funding
The authors extend their appreciation to the Researchers Supporting Program for funding this work through the Researchers Supporting Project number (RSP2023R371), King Saud University, Riyadh, Saudi Arabia.
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Z.D.: investigation, data curation, writing—original draft preparation. S.L., X.W., F.R.: visualization, investigation, writing—reviewing and editing. Shaik Althaf Hussain: conceptualization and investigation. D.J.: conceptualized, supervised, writing—reviewing and editing.
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The study has been approved by the Animal Ethical Committee of Xi’an North Hospital, China.
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Dang, Z., Liu, S., Wang, X. et al. Protective effect of avicularin against lung cancer via inhibiting inflammation, oxidative stress, and induction of apoptosis: an in vitro and in vivo study. In Vitro Cell.Dev.Biol.-Animal (2024). https://doi.org/10.1007/s11626-024-00854-x
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DOI: https://doi.org/10.1007/s11626-024-00854-x