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Drynaria Naringin alleviated mechanical stress deficiency-caused bone loss deterioration via Rspo1/Lgr4-mediated Wnt/β-catenin signalling pathway

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Abstract 

Osteoporosis is a metabolic condition distinguished by the degradation of bone microstructure and mechanical characteristics. Traditional Chinese medicine (TCM) has been employed in China for the treatment of various illnesses. Naringin, an ingredient found in Drynariae TCM, is known to have a significant impact on bone metabolism. For this research, we studied the precise potential effect of Drynaria Naringin on protecting against bone loss caused by stress deficiency. In this study, a tail-suspension (TS) test was performed to establish a mouse model with hind leg bone loss. Some mice received subcutaneous injections of Drynaria Naringin for 30 d. Trabecular bone microarchitecture was evaluated using micro-computed tomography analysis and bone histological analysis. Bone formation and resorption markers were quantified in blood samples from mice or in the supernatant of MC3T3-E1 cells by ELISA analysis, Western blotting, and PCR. Immunofluorescence was utilized to visualize the location of β-catenin. Additionally, siRNA was employed to knockdown-specific genes in the cells. Our findings highlight the efficacy of Drynaria Naringin in protecting against the deterioration of bone loss and promoting bone formation and Rspo1 expression in a mouse model following the TS test. Specifically, in vitro experiments also indicated that Drynaria Naringin may promote osteogenesis through the Wnt/β-catenin signalling pathway. Moreover, our results suggest that Drynaria Naringin upregulates the expression of Rspo1/Lgr4, leading to the promotion of osteogenesis via the Wnt/β-catenin signalling pathway. Therefore, Drynaria Naringin holds potential as a therapeutic medication for osteoporosis. Drynaria Naringin alleviates bone loss deterioration caused by mechanical stress deficiency through the Rspo1/Lgr4-mediated Wnt/β-catenin signalling pathway.

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Data availability

The data underlying this article are available from the corresponding author, Ze-Zhu Zhou.

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Funding

This work was supported by the Nature Science Foundation of Jiading district of Shanghai in China (JDKW-2020–0030).

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Author notes

  1. Gui-Xun Shi and Wei-Dong Sun contribute equally to this paper.

Authors and Affiliations

  1. Department of Orthopedic Surgery, Anting Hospital, Jiading District, Shanghai, 200805, China

    Gui-Xun Shi, Wei-Dong Sun, Chuan-Jun Yang, Wang-Lin Luo & Dan-Feng Wang

  2. Department of Anesthesiology, Putuo Central Hospital, Putuo District, Shanghai, 200333, China

    Zeng-Huan Chen

  3. Department of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, 200072, China

    Ze-Zhu Zhou

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  1. Gui-Xun Shi
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Contributions

Ze-Zhu Zhou conceived and designed the study. Gui-Xun Shi and Wei-Dong Sun performed the experiments and wrote the paper. Zeng-Huan Chen analysed the data. Chuan-Jun Yang organized the figures. Wang-Lin Luo and Dan-Feng Wang did the manuscript revision. Ze-Zhu Zhou supervised the whole study. All authors have read and approved the manuscript.

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Correspondence to Ze-Zhu Zhou.

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Shi, GX., Sun, WD., Chen, ZH. et al. Drynaria Naringin alleviated mechanical stress deficiency-caused bone loss deterioration via Rspo1/Lgr4-mediated Wnt/β-catenin signalling pathway. In Vitro Cell.Dev.Biol.-Animal 59, 706–716 (2023). https://doi.org/10.1007/s11626-023-00815-w

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