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TRIM47 promotes glioma angiogenesis by suppressing Smad4

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Abstract   

Angiogenesis is required for tumor progression; thus, its investigation can be useful to identify strategies for potential cancer treatments. Tripartite motif 47 (TRIM47) is involved in the progression of multiple cancers. However, its role in glioma angiogenesis is largely unknown. In this study, we first showed that TRIM47 is frequently upregulated in gliomas, and increased TRIM47 levels are correlated with microvascular density. We then examined the role of TRIM47 in cellular functions related to angiogenesis in vitro and observed that TRIM47 knockdown significantly reduced human umbilical vein endothelial cell proliferation, migration, and tube formation. We also found that TRIM47 silencing reduced vessel density and tumor volume in glioma xenografts. Mechanistically, TRIM47 negatively regulated Smad4 expression in glioma cells, and SMAD4 knockdown rescued the suppressive effects of TRIM47 silencing. Taken together, our results indicate that TRIM47 promotes angiogenesis in gliomas by downregulating SMAD4. Therefore, targeting the TRIM47/SMAD4 axis may offer an innovative approach to glioma treatment.

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Data availability

The data that support the findings of this study are not publicly available due to their containing information that could compromise the privacy of research participants, but are available on reasonable request from the corresponding author.

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Author notes

  1. Zifan Wang and Zhiqiang Li contributed equally to this work.

Authors and Affiliations

  1. The Affiliated Fengxian Hospital, The Central Hospital of Fengxian District) of Anhui University of Science & Technology, Shanghai Neurological Research Institute of Anhui University of Science & Technology, Shanghai, 201400, China

    Zifan Wang & Chuangchuang Han

  2. Department of Neurosurgery, The Central Hospital of Fengxian District (Shanghai Neurological Research Institute of Anhui University of Science & Technology, The Affiliated Hospital of Anhui University of Science & Technology), 6600 Nanfeng Road, Shanghai, 201400, China

    Zhiqiang Li, Yuanchi Cheng & Kaimin Wang

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  1. Zifan Wang
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  2. Zhiqiang Li
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  3. Chuangchuang Han
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  5. Kaimin Wang
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Contributions

KW, ZW, and ZL conceived and designed the study. KW, ZW, CH, and YC performed the experiment and collected, analyzed, and interpreted the data. KW wrote the manuscript. KW and ZL revised the manuscript. All of the authors read and approved the final manuscript.

Corresponding author

Correspondence to Kaimin Wang.

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Ethics approval and consent to participate

Informed consent was obtained from all individual participants included in the study. All procedures were approved by the Ethics Committee of the Central Hospital of Fengxian District and operated in keeping with the standards set out in the Announcement of Helsinki and laboratory guidelines of research in China.

Competing interests

The authors declare no competing interests.

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Wang, Z., Li, Z., Han, C. et al. TRIM47 promotes glioma angiogenesis by suppressing Smad4. In Vitro Cell.Dev.Biol.-Animal 58, 771–779 (2022). https://doi.org/10.1007/s11626-022-00722-6

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  • DOI: https://doi.org/10.1007/s11626-022-00722-6

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