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14-3-3β is essential for milk composition stimulated by Leu/IGF-1 via IGF1R signaling pathway in BMECs

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Abstract

The cell proliferation of bovine mammary epithelial cells (BMECs) and consequent milk synthesis are regulated by multiple factors. The purpose of this study was to examine the effect of 14-3-3β on cellular proliferation and milk fat/β-casein synthesis in BMECs and reveal its underlying mechanisms. In this study, we employed gene function analysis to explore the regulatory effect and molecular mechanisms of 14-3-3β on milk synthesis and proliferation in BMECs. We found that leucine and IGF-1 enhance cell proliferation and milk synthesis in a 14-3-3β-dependent manner and only exhibiting such effect in the presence of 14-3-3β. We further determined that 14-3-3β interacts with the IGF1R self-phosphorylation site and it additionally mediated leucine and IGF-1 to stimulate the synthesis of milk through the IGF1R-AKT-mTORC1 signaling pathway. In summary, our data indicated that 14-3-3β mediates the expression of milk fat and protein stimulated by leucine and IGF-1, leading to lactogenesis through IGF1R signaling pathway in BMECs.

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Acknowledgements

The authors thank all the members of the laboratory who contributed to sample determination.

Funding

This work was supported by the National Natural Science Foundation of China (31101784), “Young Talents” Project of Northeast Agricultural University (14QC43), and Heilongjiang Provincial Funding for National Subjects (GX18B023).

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Authors and Affiliations

  1. Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Hui Cao, Guan Huang, Haixin Fu & Na Zhang

Authors
  1. Hui Cao
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  2. Guan Huang
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  3. Haixin Fu
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  4. Na Zhang
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Contributions

Na Zhang contributed to this work and was the first author. Na Zhang and Hui Cao conceived and performed most of the experiments. Hui Cao and Guan Huang performed part of experiments. Na Zhang supervised the work and revised the final version of the manuscript. All authors have read and approved the final version of the manuscript.

Corresponding author

Correspondence to Na Zhang.

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Ethics approval

Relative experiments in this study were approved by the Animal Care Committee of the Northeast Agricultural University. All procedures involving dairy cows were conducted according to the Guideline for Animal Experiments of Northeast Agricultural University (Harbin, China).

Conflict of interest

The authors declare no competing interests.

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Cao, H., Huang, G., Fu, H. et al. 14-3-3β is essential for milk composition stimulated by Leu/IGF-1 via IGF1R signaling pathway in BMECs. In Vitro Cell.Dev.Biol.-Animal 58, 384–395 (2022). https://doi.org/10.1007/s11626-022-00682-x

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