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miR-153-3p inhibited osteogenic differentiation of human DPSCs through CBFβ signaling

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Abstract

Dental pulp stem cells (DPSCs) have multilineage differentiation potential and especially show a great foreground in bone regeneration engineering. The mechanism of osteogenic differentiation of DPSCs needs to be explored exactly. As a kind of endogenous and non-coding small RNAs, microRNAs (miRNAs) play an important role in many biological processes including osteogenic differentiation. However, the mechanism of miR-153-3p in osteogenic differentiation of DPSCs is still unknown. Core-binding factors-beta (CBFβ) is a non-DNA-binding factor that combines with the runt-related transcription factor family transcription factors to mediate their DNA-binding affinities, and plays a critical role in regulating osteogenic differentiation. In this study, we explored the mechanisms of miR-153-3p and CBFβ in DPSC osteogenesis. The expression of miR-153-3p and CBFβ was tested under the osteogenic condition, and the influence led by changing the expression of miR-153-3p or CBFβ had also been detected. A luciferase reporter assay confirmed that miR-153-3p directly targeted to CBFβ. The osteogenic markers, alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and bone morphogenetic protein 2 (BMP2), were tested in protein level or mRNA level. ALP and Alizarin red staining were used to detect the osteoblast activity and mineral deposition. In osteogenic condition, the expressions of CBFβ and osteogenic markers were upregulated, whereas that of miR-153-3p was downregulated. miR-153-3p negatively regulated the osteogenic differentiation, and overexpression of CBFβ could offset the negative effect of miR-153-3p. Our findings provided a novel strategy for DPSC application in treatment of bone deficiencies and facilitated bone regeneration.

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Funding

This work was supported by Science Foundation of Suzhou Stomatological Hospital (SZKQYY20A001), and Suzhou Science and Technology Development Program (SYSD2020071).

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Author notes

  1. Changbo Wei and Manru Chu contributed equally to this work

Authors and Affiliations

  1. The Affiliated Stomatological Hospital of Soochow University, Suzhou Stomatological Hospital, Suzhou, 215000, China

    Changbo Wei & Manru Chu

  2. Department of Stomatology, Wuxi No 2 People’s Hospital, Wuxi, 214000, China

    Ke Zheng & Ping He

  3. Department of Stomatology, Haimen People’s Hospital Affiliated To Nantong University, Nantong, 226199, China

    Jingwen Xiao

  4. Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, China

    Jingwen Xiao

Authors
  1. Changbo Wei
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  2. Manru Chu
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  3. Ke Zheng
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  4. Ping He
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  5. Jingwen Xiao
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Corresponding author

Correspondence to Jingwen Xiao.

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Our study was approved by the Ethics Committee of the Affiliated Stomatological Hospital of Soochow University.

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The authors declare that they have no conflicts of interest.

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Wei, C., Chu, M., Zheng, K. et al. miR-153-3p inhibited osteogenic differentiation of human DPSCs through CBFβ signaling. In Vitro Cell.Dev.Biol.-Animal 58, 316–324 (2022). https://doi.org/10.1007/s11626-022-00665-y

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  • DOI: https://doi.org/10.1007/s11626-022-00665-y

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