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Optimization of porcine embryonic germ cell culture system

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Abstract

Homologous feeder culture system can efficiently promote the proliferation of embryonic germ (EG) cells or embryonic stem (ES) cells while avoiding contamination by exogenous proteins and pathogens. In this study, we compared the potency of using homologous porcine embryonic fibroblasts (PEFs), gonadal stromal cells (GSCs), porcine adipose–derived stem cells (PASCs), or porcine amniotic fluid stem (PAFS) cells as feeder cells for porcine EG growth, with the commonly used mouse embryonic fibroblasts (MEFs). We compared the feeder cell growth rates; secretion of growth factors including stem cell factor (SCF), basic fibroblast growth factor (bFGF), and leukemia inhibitory factor (LIF); the effects of growth factors on porcine PGC growth; and EG growth rates when individual cells were used as feeders. Our results showed that feeder cells secreted limited amounts of growth factors, and supplementation of growth factors can significantly improve the formation of EG colonies and number of passages (P < 0.05). GSC and PEF were more suitable for EG growth because of their faster growth rate and their support on EG growth. In conclusion, this study identified novel homologous cells that can be used for EG production.

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Acknowledgments

The authors thank Minjun Cao for the technical support.

Funding

This work was supported by the High-level Talent Scientific Research Foundation of Qingdao Agricultural University (No.6631117017).

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Author notes

  1. Yang Li and Yu Qiao contributed equally to this work.

Authors and Affiliations

  1. College of Life Science, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, 266109, Shandong, People’s Republic of China

    Yang Li, Yu Qiao, Fei Li & Xiao Dong

  2. Department of Surgery,, Medical University of South Carolina, Charleston, SC, 29425, USA

    Hongjun Wang

Authors
  1. Yang Li
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  2. Yu Qiao
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  3. Fei Li
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  4. Hongjun Wang
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  5. Xiao Dong
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Corresponding author

Correspondence to Xiao Dong.

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The authors declare that they have no conflict of interest.

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Editor: Tetsuji Okamoto

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Li, Y., Qiao, Y., Li, F. et al. Optimization of porcine embryonic germ cell culture system. In Vitro Cell.Dev.Biol.-Animal 56, 808–815 (2020). https://doi.org/10.1007/s11626-020-00489-8

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  • DOI: https://doi.org/10.1007/s11626-020-00489-8

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