YY1-induced upregulation of lncRNA NEAT1 contributes to OGD/R injury-induced inflammatory response in cerebral microglial cells via Wnt/β-catenin signaling pathway

  • Dong HanEmail author
  • Yidong Zhou


Stroke can lead to the serious long-term neurological disability. The dysregulation of long non-coding RNAs (lncRNAs) has been proven to be a pivotal factor for the progression of ischemic stroke. However, it is largely unknown whether lncRNAs regulated the OGD/R injury of cerebral microglial cells. In this study, we designed experiments to reveal the role of lncRNA Nuclear Enriched Abundant Transcript 1 (NEAT1) in the OGD/R injury of microglial cells. We found that NEAT1 contributed to the OGD/R injury and neuroinflammation damage in microglial cells. Moreover, the molecular mechanism involved in the NEAT1-mediated OGD/R injury. Mechanism investigation revealed that NEAT1 was upregulated by the transcription factor YY1. Moreover, Western blot analysis suggested that NEAT1 enhance the protein levels of core factors of Wnt/β-catenin signaling pathway, indicating that NEAT1 contributed to the activation of Wnt/β-catenin signaling pathway. Rescue assays were carried out in the microglial cells treated with OGD/R. The results showed that NEAT1 regulated the OGD/R injury and neuroinflammation damage via Wnt/β-catenin signaling pathway. In conclusion, our findings suggested that YY1-induced upregulation of NEAT1 contributed to the OGD/R injury and neuroinflammation damage of microglial cells via Wnt/β-catenin signaling pathway.


YY1 lncRNA NEAT1 OGD/R injury Microglial cells Wnt/β-catenin signaling pathway 



The authors sincerely appreciate all lab members.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

11626_2019_375_Fig6_ESM.png (43 kb)

The expression of NEAT1 was reduced by transfection of si-NEAT#1/2/3. (PNG 42 kb)

11626_2019_375_MOESM1_ESM.tif (1.3 mb)
High resolution image (TIF 1304 kb)
11626_2019_375_MOESM2_ESM.xlsx (9 kb)
ESM 2 (XLSX 9 kb)


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Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.Department of Neurology, Ningbo first hospitalNingboChina

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