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Unexpected dose response of HaCaT to UVB irradiation

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Abstract

Application of high-dosage UVB irradiation in phototherapeutic dermatological treatments present health concerns attributed to UV-exposure. In assessing UV-induced photobiological damage, we investigated dose-dependent effects of UVB irradiation on human keratinocyte cells (HaCaT). Our study implemented survival and apoptosis assays and revealed an unexpected dose response wherein higher UVB-dosage induced higher viability. Established inhibitors, such as AKT− (LY294002), PKC− (Gö6976, and Rottlerin), ERK− (PD98059), P38 MAPK− (SB203580), and JNK− (SP600125), were assessed to investigate UV-induced apoptotic pathways. Despite unobvious contributions of known signaling pathways in dose-response mediation, microarray analysis identified transcriptional expression of UVB-response genes related to the respiratory-chain. Observed correlation of ROS-production with UVB irradiation potentiated ROS as the underlying mechanism for observed dose responses. Inability of established pathways to explain such responses suggests the complex nature underlying UVB-phototherapy response.

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Funding

The work was supported by grants from the Ministry of Science and Technology, Taiwan (MOST 104-2112-M-007-016). C.S.C was supported by the Ministry of Science and Technology, Taiwan (MOST 105-2112-M-007-010).

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Authors and Affiliations

  1. Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Section2, Kuang-Fu Road, Hsinchu, 30013, Taiwan

    Rong-Shing Chang, Chi-Shuo Chen, Ching-Lung Huang, Chiu-Ting Chang, Yujia Cui, Chi-Shiun Chiang, Chun-Yu Chuang & Ian C. Hsu

  2. Institute of Statistics, National Tsing Hua University, Hsinchu, Taiwan

    Wun-Yi Shu

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  1. Rong-Shing Chang
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Correspondence to Ian C. Hsu.

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Editor: Tetsuji Okamoto

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Chang, RS., Chen, CS., Huang, CL. et al. Unexpected dose response of HaCaT to UVB irradiation. In Vitro Cell.Dev.Biol.-Animal 54, 589–599 (2018). https://doi.org/10.1007/s11626-018-0280-4

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  • DOI: https://doi.org/10.1007/s11626-018-0280-4

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