Abstract
Wolbachia pipientis (Rickettsiales; Anaplasmataceae) is an obligate intracellular alpha proteobacterium that occurs in arthropods and filarial worms. Some strains of Wolbachia can be maintained as persistent infections in insect cell lines. C/wStr1 cells from the mosquito Aedes albopictus maintain a robust infection with Wolbachia strain wStr, originally isolated from the planthopper, Laodelphax striatellus. To explore possible functions of penicillin-binding proteins expressed from the wStr genome, C/wStr1 cells were exposed to ampicillin. Absolute levels of Wolbachia increased 3.5-fold in ampicillin-treated cells and fivefold in naive cells newly infected with wStr. Because cell numbers were depressed by ampicillin treatment, Wolbachia yield on a per-cell basis increased by 15-fold. The absence of a similar effect on wAlbB in Aa23 host cells suggests that the Wolbachia strain, the presence/absence of genes encoding penicillin-binding proteins, or the interaction between wAlbB and its host cells may modulate the effects of ampicillin.
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This work was supported by NIH grant AI081322 and by the University of Minnesota Agricultural Experiment Station, St. Paul, MN.
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Fallon, A.M. Strain-specific response to ampicillin in Wolbachia-infected mosquito cell lines. In Vitro Cell.Dev.Biol.-Animal 54, 580–588 (2018). https://doi.org/10.1007/s11626-018-0279-x
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DOI: https://doi.org/10.1007/s11626-018-0279-x