The effect of storage temperature on the biological activity of extracellular vesicles for the complement system

Abstract

Extracellular vesicles (EVs) are mediators of intercellular communication by transporting cargo containing proteins, lipids, mRNA, and miRNA. There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have found that EVs from Kaposi’s sarcoma-associated herpesvirus (KSHV)-infected human endothelial cells have the potential to activate the complement system. Although many studies have shown that the physical properties of EVs can be changed by their storage condition, there have been few studies for the stability of biological activity of EVs in various storage conditions. In this study, we investigated various conditions to identify the best conditions to store EVs with functional stability for 25 d. Furthermore, the correlation between the function and other characteristics of EVs, including the expression of EV markers, size distribution, and particle number, were also analyzed. Our results demonstrated that storage temperature is an important factor to maintain the activity of EVs and would be useful information for basic research and clinical application using EVs.

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Funding

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF-2017R1A2B4002405). SJP is partially supported by a fellowship from Eulji University Graduate School.

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Correspondence to Myung-Shin Lee.

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Editor: Tetsuji Okamoto

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Park, S.J., Jeon, H., Yoo, S. et al. The effect of storage temperature on the biological activity of extracellular vesicles for the complement system. In Vitro Cell.Dev.Biol.-Animal 54, 423–429 (2018). https://doi.org/10.1007/s11626-018-0261-7

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Keywords

  • Extracellular vesicles
  • Exosome
  • Complement system
  • KSHV
  • Storage
  • Stability
  • Temperature