The effect of storage temperature on the biological activity of extracellular vesicles for the complement system
- 234 Downloads
Extracellular vesicles (EVs) are mediators of intercellular communication by transporting cargo containing proteins, lipids, mRNA, and miRNA. There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have found that EVs from Kaposi’s sarcoma-associated herpesvirus (KSHV)-infected human endothelial cells have the potential to activate the complement system. Although many studies have shown that the physical properties of EVs can be changed by their storage condition, there have been few studies for the stability of biological activity of EVs in various storage conditions. In this study, we investigated various conditions to identify the best conditions to store EVs with functional stability for 25 d. Furthermore, the correlation between the function and other characteristics of EVs, including the expression of EV markers, size distribution, and particle number, were also analyzed. Our results demonstrated that storage temperature is an important factor to maintain the activity of EVs and would be useful information for basic research and clinical application using EVs.
KeywordsExtracellular vesicles Exosome Complement system KSHV Storage Stability Temperature
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF-2017R1A2B4002405). SJP is partially supported by a fellowship from Eulji University Graduate School.
- Maroto R, Zhao Y, JamaluddinM, Popov VL,Wang H, KalubowilageM, Zhang Y, Luisi J, Sun H, Culbertson CT, Bossmann SH, Motamedi M, Brasier AR (2017) Effects of storage temperature on airway 342 exosome integrity for diagnostic and functional analyses. J 343 Extracell Vesicles 6:1359478Google Scholar
- Roma-Rodrigues C, Fernandes AR, Baptista PV (2014) Exosome in tumour microenvironment: overview of the crosstalk between normal and cancer cells. Biomed Res Int 2014:179486Google Scholar
- Zhao H, Yang L, Baddour J, Achreja A, Bernard V, Moss T, Marini JC, Tudawe T, Seviour EG, San Lucas FA, Alvarez H, Gupta S, Maiti SN, Cooper L, Peehl D, Ram PT, Maitra A, Nagrath D (2016) Tumor microenvironment derived exosomes pleiotropically modulate cancer cell metabolism. Elife 5:e10250PubMedPubMedCentralCrossRefGoogle Scholar