Isolation and identification of bovine nasopharyngeal mucosal epithelial cells and establishment of cell models of acute infection by foot-and-mouth disease virus
- 141 Downloads
Foot-and-mouth disease (FMD) commonly occurs via the respiratory tract, and bovine nasopharyngeal mucosal epithelial cells are the primary infection cells in cattle. The aim of the present study was to isolate and culture epithelial cells from the bovine nasopharyngeal mucosa in vitro using a mechanical separation method. The cells were expanded, established in continuous cell culture, and used for immunofluorescence cytochemistry and establishment of infection models. We detected pan-cytokeratin markers of bovine nasopharyngeal mucosal epithelial cells by immunofluorescence. Bovine nasopharyngeal mucosal epithelial cells were then infected with foot-and-mouth disease virus (FMDV) serum type O. RT-PCR demonstrated the successful establishment of acute FMDV infection in the cell models. This infection model provides the basis for clarification of the interaction between FMDV and host bovine nasopharyngeal mucosal epithelial cells in vitro.
KeywordsBovine nasopharyngeal mucosal epithelial cells Cell isolation Cell identification Infection models FMDV
We sincerely acknowledge the help of all the members of Research and Development Department of Inner Mongolia Bigvet Biotech Co., Ltd. We thank Dr. Eerdunmutu (Inner Mongolia Agricultural University) for sample collection and discussions.
Compliance with ethical standards
Animals were sacrificed in accordance with the guidelines of the Ethics Committee of the Institute of Inner Mongolia Agricultural University.
Conflict of interest
The authors declare that they have no conflict of interest.
- Alexandersen S, Mowat N (2005) Foot-and-mouth disease: host range and pathogenesis. Curr Top Microbial Immunol 288:9–42Google Scholar
- Mullol J, Roca-Ferrer J, Alobid I, Pujols L, Valero A, Xaubet A, Bernal-Sprekelsen M, Picado C (2006) Effect of desloratadine on epithelial cell granulocyte-macrophage colony-stimulating factor secretion and eosinophil survival. Clin Exp Allergy 36(1):52–58. https://doi.org/10.1111/j.1365-2222.2005.02403.x CrossRefPubMedGoogle Scholar
- Pacheco JM, Smoliga GR, O'Donnell V, Brito BP, Stenfeldt C, Rodriguez LL, Arzt J (2015) Persistent foot-and-mouth disease virus infection in the nasopharynx of cattle; tissue-specific distribution and local cytokine expression. PLoS One 10(5):e0125698. https://doi.org/10.1371/journal.pone.0125698 CrossRefPubMedPubMedCentralGoogle Scholar
- Stenfeldt C, Belsham GJ (2012) Detection of foot-and-mouth disease virus RNA in pharyngeal epithelium biopsy samples obtained from infected cattle: investigation of possible sites of virus replication and persistence. Vet Microbiol 154(3-4):230–239. https://doi.org/10.1016/j.vetmic.2011.07.007 CrossRefPubMedGoogle Scholar
- Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015) Pathogenesis of primary foot-and-mouth disease virus infection in the nasopharynx of vaccinated and non-vaccinated cattle. PLoS One 10(11):e0143666. https://doi.org/10.1371/journal.pone.0143666 CrossRefPubMedPubMedCentralGoogle Scholar